Mycoplasma pneumoniae CARDS toxin exacerbates ovalbumin-induced asthma-like inflammation in BALB/c mice

Jorge L. Medina, Jacqueline J. Coalson, Edward G Brooks, Claude T Chapman, Vicki T. Winter, Adriana Chaparro, Molly F R Principe, Laura Solis, Thirumalai R Kannan, Joel B. Baseman, Peter H Dube

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Mycoplasma pneumoniae causes a range of airway and extrapulmonary pathologies in humans. Clinically, M. pneumoniae is associated with acute exacerbations of human asthma and a worsening of experimentally induced asthma in mice. Recently, we demonstrated that Community Acquired Respiratory Distress Syndrome (CARDS) toxin, an ADP-ribosylating and vacuolating toxin synthesized by M. pneumoniae, is sufficient to induce an asthma-like disease in BALB/cJ mice. To test the potential of CARDS toxin to exacerbate preexisting asthma, we examined inflammatory responses to recombinant CARDS toxin in an ovalbumin (OVA) murine model of asthma. Differences in pulmonary inflammatory responses between treatment groups were analyzed by histology, cell differentials and changes in cytokine and chemokine concentrations. Additionally, assessments of airway hyperreactivity were evaluated through direct pulmonary function measurements. Analysis of histology revealed exaggerated cellular inflammation with a strong eosinophilic component in the CARDS toxintreated group. Heightened T-helper type-2 inflammatory responses were evidenced by increased expression of IL-4, IL-13, CCL17 and CCL22 corresponding with increased airway hyperreactivity in the CARDS toxin-treated mice. These data demonstrate that CARDS toxin can be a causal factor in the worsening of experimental allergic asthma, highlighting the potential importance of CARDS toxin in the etiology and exacerbation of human asthma.

Original languageEnglish (US)
Article numbere102613
JournalPLoS One
Volume9
Issue number7
DOIs
StatePublished - Jul 24 2014

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Mycoplasma pneumoniae
ovalbumin
Ovalbumin
asthma
distress
Histology
toxins
Asthma
inflammation
Inflammation
mice
Interleukin-13
Pathology
Chemokines
Interleukin-4
Adenosine Diphosphate
histology
Cytokines
Lung
lung function

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Mycoplasma pneumoniae CARDS toxin exacerbates ovalbumin-induced asthma-like inflammation in BALB/c mice. / Medina, Jorge L.; Coalson, Jacqueline J.; Brooks, Edward G; Chapman, Claude T; Winter, Vicki T.; Chaparro, Adriana; Principe, Molly F R; Solis, Laura; Kannan, Thirumalai R; Baseman, Joel B.; Dube, Peter H.

In: PLoS One, Vol. 9, No. 7, e102613, 24.07.2014.

Research output: Contribution to journalArticle

Medina, JL, Coalson, JJ, Brooks, EG, Chapman, CT, Winter, VT, Chaparro, A, Principe, MFR, Solis, L, Kannan, TR, Baseman, JB & Dube, PH 2014, 'Mycoplasma pneumoniae CARDS toxin exacerbates ovalbumin-induced asthma-like inflammation in BALB/c mice', PLoS One, vol. 9, no. 7, e102613. https://doi.org/10.1371/journal.pone.0102613
Medina, Jorge L. ; Coalson, Jacqueline J. ; Brooks, Edward G ; Chapman, Claude T ; Winter, Vicki T. ; Chaparro, Adriana ; Principe, Molly F R ; Solis, Laura ; Kannan, Thirumalai R ; Baseman, Joel B. ; Dube, Peter H. / Mycoplasma pneumoniae CARDS toxin exacerbates ovalbumin-induced asthma-like inflammation in BALB/c mice. In: PLoS One. 2014 ; Vol. 9, No. 7.
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