Mutatsii, predeterminirovannye pervichnoǐ strukturoǐ DNK.

Translated title of the contribution: Mutations predetermined by the primary structure of DNA

R. I. Salganik, G. L. Dianov, A. V. Mazin

Research output: Contribution to journalArticlepeer-review

Abstract

This study is concerned with an experimental verification of hypotheses postulating the involvement of self-complementary nucleotide sequences in the formation of deletions and insertions. It was suggested that deletions can arise in the regions of self-complementary nucleotide sequences, which allows the formation of the hairpin structures in a single-stranded DNA, arising during excision repair. These hairpin structures can be eliminated by nucleases or during DNA replication. Insertions can arise as a result of homologous recombination, when a migrating DNA strand contains a self-complementary sequence which forms hairpin structure. Model experiments were carried out with the pBR322 plasmid. A plasmid DNA with premutational damage in the palindrome-containing region was constructed by in vitro dimethylsulfate modification of one strand of EcoRI-BamHI restriction fragment. The plasmid was used for transformation of Escherichia coli. Restriction mapping and nucleotide analysis of the mutant DNAs demonstrated that they all contained deletions. The end points of the deletions coincide with the palindrome. To model homologous recombination, a plasmid with D-loop was constructed. A single-stranded DNA fragment containing palindrome forming a hairpin structure was introduced into the plasmid DNA and covalently fixed in the complex. When E. coli cells were transfected with this DNA, plasmid mutants containing insertions predetermined by palindromic structure arose. The evolutionary role of mutations predetermined by primary DNA structure is discussed.

Translated title of the contributionMutations predetermined by the primary structure of DNA
Original languageRussian
Pages (from-to)2398-2407
Number of pages10
JournalGenetika
Volume22
Issue number10
StatePublished - Oct 1986
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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