Mutations of the withered (whd) gene in Drosophila melanogaster confer hypersensitivity to oxidative stress and are lesions of the carnitine palmitoyltransferase I (CPT I) gene

Benjamin R. Strub, Tony L. Parkes, Spencer T. Mukai, Sepehr Bahadorani, Alistair B. Coulthard, Nichole Hall, John P. Phillips, Arthur J. Hilliker

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Since some oxygen defense mutants of Drosophila melanogaster exhibit a crinkled wing phenotype, a screen was performed on strains bearing mutant alleles conferring a visible wing phenotype to determine whether any were hypersensitive to oxidative stress. One mutant, withered (whd), was found to be sensitive to both dietary paraquat and hyperoxia. New alleles of whd were induced on a defined genetic background and strains carrying these alleles were also found to be sensitive to oxidative stress. To identify the product of the whd gene we used a sequence-based positional candidate approach and by this method we determined that whd encodes carnitine palmitoyltransferase I (CPT I), an enzyme of the outer mitochondrial membrane that is required for the import of long-chain fatty acids into the mitochondria for β-oxidation. Although this function is not vital under laboratory conditions, whd adults were found to be highly sensitive to starvation and to heavy metal toxicity relative to controls. This work uncovers a novel relationship between fatty acid metabolism and reactive oxygen metabolism. Further, these results in conjunction with past research on whd and on mammalian CPT I support the hypothesis that CPT I serves a vital function in the response to thymine supplementation.

Original languageEnglish (US)
Pages (from-to)409-420
Number of pages12
JournalGenome
Volume51
Issue number6
DOIs
StatePublished - Jun 2008
Externally publishedYes

Keywords

  • Carnitine palmitoyltransferase I
  • Drosophila melanogaster
  • Fatty acid
  • Oxidative stress
  • Thymine

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics

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