Mutational analysis of the capsid protein of Leishmania RNA virus LRV1-4

Tamarra L. Cadd, Kyle Macbeth, Dierdre Furlong, Jean L. Patterson

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The virion of Leishmania RNA virus is predicted to be composed of a 742- amino-acid major capsid protein and a small percentage of capsid-polymerase fusion molecules. Recently, the capsid protein alone was expressed and shown to spontaneously assemble into viruslike particles. Since the major structural protein of the virion shell self-assembles into viruslike particles when expressed in the baculovirus expression system, assembly of the virion can be studied by mutational analysis and expression of a single open reading frame. In this study, several deletions and one addition of the capsid protein of Leishmania RNA virus LRV1-4 were generated. These mutants show different degrees of assembly. Assembly domains are being identified such that the capsid protein may be used as a macromolecular packaging and delivery system for Leishmania species.

Original languageEnglish (US)
Pages (from-to)7738-7745
Number of pages8
JournalJournal of virology
Volume68
Issue number12
DOIs
StatePublished - Dec 1994
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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