Mutational analysis of the capsid protein of Leishmania RNA virus LRV1-4

Tamarra L. Cadd, Kyle Macbeth, Dierdre Furlong, Jean L. Patterson

    Research output: Contribution to journalArticlepeer-review

    7 Scopus citations

    Abstract

    The virion of Leishmania RNA virus is predicted to be composed of a 742- amino-acid major capsid protein and a small percentage of capsid-polymerase fusion molecules. Recently, the capsid protein alone was expressed and shown to spontaneously assemble into viruslike particles. Since the major structural protein of the virion shell self-assembles into viruslike particles when expressed in the baculovirus expression system, assembly of the virion can be studied by mutational analysis and expression of a single open reading frame. In this study, several deletions and one addition of the capsid protein of Leishmania RNA virus LRV1-4 were generated. These mutants show different degrees of assembly. Assembly domains are being identified such that the capsid protein may be used as a macromolecular packaging and delivery system for Leishmania species.

    Original languageEnglish (US)
    Pages (from-to)7738-7745
    Number of pages8
    JournalJournal of virology
    Volume68
    Issue number12
    DOIs
    StatePublished - Dec 1994

    ASJC Scopus subject areas

    • Microbiology
    • Immunology
    • Insect Science
    • Virology

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