Mutation in the TP53 gene in colorectal carcinoma detected by polymerase chain reaction

Eun‐Soo ‐S Han, Mary Pat Moyer, Susan Naylor, Alan Y. Sakaguchi

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The human TP53 gene is a possible tumor suppressor since TP53 gene mutations are observed in >70% of sporadic colorectal carcinoma DNAs. In genomic DNAs from seven colon cancer cell samples, a 405 base pair DNA fragment containing exon 5, intron 5, and exon 6 of the TP53 gene was amplified by polymerase chain reaction and analyzed for mutations. One sample [human colon cancer (HCC) 278] was found to have a TP53 mutation altering the amino acid glutamine 167 in exon 5. A deletion of 2 bases changed glutamine 167 (CAG) to alanine (GCA) and the resulting frame‐shift produced an in‐frame stop codon at amino acid 179. While the normal TP53 gene gives rise to a 53 kD protein, the estimated size of this mutant TP53 protein if expressed would be approximately 20 kD.

Original languageEnglish (US)
Pages (from-to)313-317
Number of pages5
JournalGenes, Chromosomes and Cancer
Volume3
Issue number4
DOIs
StatePublished - Jul 1991

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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