Mustard NPR1, a mammalian IκB homologue inhibits NF-κB activation in human GBM cell lines

Divya Kesanakurti, Gangadhara Reddy Sareddy, Phanithi Prakash Babu, Pulugurtha Bharadwaja Kirti

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

NF-κB activity is tightly regulated by IκB class of proteins. IκB proteins possess ankyrin repeats for binding to and inhibiting NF-κB. The regulatory protein, NPR1 from Brassica juncea possesses ankyrin repeats with sequence similarity to IκBα subgroup. Therefore, we examined whether stably expressed BjNPR1 could function as IκB in inhibiting NF-κB in human glioblastoma cell lines. We observed that BjNPR1 bound to NF-κB and inhibited its nuclear translocation. Further, BjNPR1 expression down-regulated the NF-κB target genes iNOS, Cox-2, c-Myc and cyclin D1 and reduced the proliferation rate of U373 cells. Finally, BjNPR1 decreased the levels of pERK, pJNK and PKCα and increased the Caspase-3 and Caspase-8 activities. These results suggested that inhibition of NF-κB activation by BjNPR1 can be a promising therapy in NF-κB dependent pathologies.

Original languageEnglish (US)
Pages (from-to)427-433
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume390
Issue number3
DOIs
StatePublished - Dec 18 2009

Keywords

  • IκB
  • Mustard
  • NF-κB
  • NPR1
  • U373
  • p50
  • p65

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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