Musashi 1 modulates cell proliferation genes in the medulloblastoma cell line Daoy

Patricia C. Sanchez-Diaz, Tarea L. Burton, Suzanne C. Burns, Jaclyn Y. Hung, Luiz O Penalva

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Abstract

Background: Musashi1 (Msi1) is an RNA binding protein with a central role during nervous system development and stem cell maintenance. High levels of Msi1 have been reported in several malignancies including brain tumors thereby associating Msi1 and cancer. Methods: We used the human medulloblastoma cell line Daoy as model system in this study to knock down the expression of Msi1 and determine the effects upon soft agar growth and neurophere formation. Quantitative RT-PCR was conducted to evaluate the expression of cell proliferation, differentiation and survival genes in Msi1 depleted Daoy cells. Results: We observed that MSI1 expression was elevated in Daoy cells cultured as neurospheres compared to those grown as monolayer. These data indicated that Msi1 might be involved in regulating proliferation in cancer cells. Here we show that shRNA mediated Msi1 depletion in Daoy cells notably impaired their ability to form colonies in soft agar and to grow as neurospheres in culture. Moreover, differential expression of a group of Notch, Hedgehog and Wnt pathway related genes including MYCN, FOS, NOTCH2, SMO, CDKN1A, CCND2, CCND1, and DKK1, was also found in the Msi1 knockdown, demonstrating that Msi1 modulated the expression of a subset of cell proliferation, differentiation and survival genes in Daoy. Conclusion: Our data suggested that Msi1 may promote cancer cell proliferation and survival as its loss seems to have a detrimental effect in the maintenance of medulloblastoma cancer cells. In this regard, Msi1 might be a positive regulator of tumor progression and a potential target for therapy.

Original languageEnglish (US)
Article number280
JournalBMC Cancer
Volume8
DOIs
StatePublished - Sep 30 2008

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Medulloblastoma
Cell Proliferation
Cell Line
Genes
Neoplasms
Cell Survival
Agar
Cell Differentiation
Maintenance
Wnt Signaling Pathway
RNA-Binding Proteins
Brain Neoplasms
Nervous System
Small Interfering RNA
Cultured Cells
Stem Cells
Polymerase Chain Reaction
Growth

ASJC Scopus subject areas

  • Oncology
  • Cancer Research
  • Genetics

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Musashi 1 modulates cell proliferation genes in the medulloblastoma cell line Daoy. / Sanchez-Diaz, Patricia C.; Burton, Tarea L.; Burns, Suzanne C.; Hung, Jaclyn Y.; Penalva, Luiz O.

In: BMC Cancer, Vol. 8, 280, 30.09.2008.

Research output: Contribution to journalArticle

Sanchez-Diaz, Patricia C. ; Burton, Tarea L. ; Burns, Suzanne C. ; Hung, Jaclyn Y. ; Penalva, Luiz O. / Musashi 1 modulates cell proliferation genes in the medulloblastoma cell line Daoy. In: BMC Cancer. 2008 ; Vol. 8.
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