Mus spretus LINE-1 sequences detected in the Mus musculus inbred strain C57BL/6J using LINE-1 DNA probes

B. A. Rikke, Y. Zhao, L. P. Daggett, R. Reyes, S. C. Hardies

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The inbred mouse strain, C57BL/6J, was derived from mice of the Mus musculus complex, C57BL/6J can be crossed in the laboratory with a closely related mouse species, M. spretus to produce fertile offspring; however there has been no previous evidence of gene flow between M. spretus and M. musculus in nature. Analysis of the repetitive sequence LINE-1, using both direct sequence analysis and genomic Southern blot hybridization to species-specific LINE-1 hybridization probes, demonstrates the presence of LINE-1 elements in C57BL/6J that were derived form the species M. spretus. These spretus-like LINE-1 elements in C57BL/6J reveal a cross to M. spretus somewhere in the history of C57BL/6J. It is unclear if the spretus-like LINE-1 elements are still embedded in flanking DNA derived from M. spretus or if they have transposed to new sites. The number of spretus-like elements detected suggests a maximum of 6.5% of the C57BL/6J genome may be derived from M. spretus.

Original languageEnglish (US)
Pages (from-to)901-906
Number of pages6
JournalGenetics
Volume139
Issue number2
StatePublished - 1995

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Long Interspersed Nucleotide Elements
DNA Probes
Inbred Strains Mice
Gene Flow
Nucleic Acid Repetitive Sequences
Southern Blotting
Sequence Analysis
History
Genome
DNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Rikke, B. A., Zhao, Y., Daggett, L. P., Reyes, R., & Hardies, S. C. (1995). Mus spretus LINE-1 sequences detected in the Mus musculus inbred strain C57BL/6J using LINE-1 DNA probes. Genetics, 139(2), 901-906.

Mus spretus LINE-1 sequences detected in the Mus musculus inbred strain C57BL/6J using LINE-1 DNA probes. / Rikke, B. A.; Zhao, Y.; Daggett, L. P.; Reyes, R.; Hardies, S. C.

In: Genetics, Vol. 139, No. 2, 1995, p. 901-906.

Research output: Contribution to journalArticle

Rikke, BA, Zhao, Y, Daggett, LP, Reyes, R & Hardies, SC 1995, 'Mus spretus LINE-1 sequences detected in the Mus musculus inbred strain C57BL/6J using LINE-1 DNA probes', Genetics, vol. 139, no. 2, pp. 901-906.
Rikke BA, Zhao Y, Daggett LP, Reyes R, Hardies SC. Mus spretus LINE-1 sequences detected in the Mus musculus inbred strain C57BL/6J using LINE-1 DNA probes. Genetics. 1995;139(2):901-906.
Rikke, B. A. ; Zhao, Y. ; Daggett, L. P. ; Reyes, R. ; Hardies, S. C. / Mus spretus LINE-1 sequences detected in the Mus musculus inbred strain C57BL/6J using LINE-1 DNA probes. In: Genetics. 1995 ; Vol. 139, No. 2. pp. 901-906.
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