Mus spretus LINE-1 sequences detected in the Mus musculus inbred strain C57BL/6J using LINE-1 DNA probes

B. A. Rikke, Y. Zhao, L. P. Daggett, R. Reyes, S. C. Hardies

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The inbred mouse strain, C57BL/6J, was derived from mice of the Mus musculus complex, C57BL/6J can be crossed in the laboratory with a closely related mouse species, M. spretus to produce fertile offspring; however there has been no previous evidence of gene flow between M. spretus and M. musculus in nature. Analysis of the repetitive sequence LINE-1, using both direct sequence analysis and genomic Southern blot hybridization to species-specific LINE-1 hybridization probes, demonstrates the presence of LINE-1 elements in C57BL/6J that were derived form the species M. spretus. These spretus-like LINE-1 elements in C57BL/6J reveal a cross to M. spretus somewhere in the history of C57BL/6J. It is unclear if the spretus-like LINE-1 elements are still embedded in flanking DNA derived from M. spretus or if they have transposed to new sites. The number of spretus-like elements detected suggests a maximum of 6.5% of the C57BL/6J genome may be derived from M. spretus.

Original languageEnglish (US)
Pages (from-to)901-906
Number of pages6
JournalGenetics
Volume139
Issue number2
StatePublished - Jan 1 1995

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Mus spretus LINE-1 sequences detected in the Mus musculus inbred strain C57BL/6J using LINE-1 DNA probes'. Together they form a unique fingerprint.

Cite this