Murine model of intestinal ischemia-reperfusion injury

Ekaterina O. Gubernatorova, Ernesto Perez-Chanona, Ekaterina P. Koroleva, Christian Jobin, Alexei V. Tumanov

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Intestinal ischemia is a life-threatening condition associated with a broad range of clinical conditions including atherosclerosis, thrombosis, hypotension, necrotizing enterocolitis, bowel transplantation, trauma and chronic inflammation. Intestinal ischemia-reperfusion (IR) injury is a consequence of acute mesenteric ischemia, caused by inadequate blood flow through the mesenteric vessels, resulting in intestinal damage. Reperfusion following ischemia can further exacerbate damage of the intestine. The mechanisms of IR injury are complex and poorly understood. Therefore, experimental small animal models are critical for understanding the pathophysiology of IR injury and the development of novel therapies. Here we describe a mouse model of acute intestinal IR injury that provides reproducible injury of the small intestine without mortality. This is achieved by inducing ischemia in the region of the distal ileum by temporally occluding the peripheral and terminal collateral branches of the superior mesenteric artery for 60 min using microvascular clips. Reperfusion for 1 hr, or 2 hr after injury results in reproducible injury of the intestine examined by histological analysis. Proper position of the microvascular clips is critical for the procedure. Therefore the video clip provides a detailed visual step-by-step description of this technique. This model of intestinal IR injury can be utilized to study the cellular and molecular mechanisms of injury and regeneration.

Original languageEnglish (US)
Article numbere53881
JournalJournal of Visualized Experiments
Volume2016
Issue number111
DOIs
StatePublished - May 11 2016
Externally publishedYes

Keywords

  • Intestinal injury
  • Ischemia
  • Issue 111
  • Laparotomy
  • Medicine
  • Mouse
  • Regeneration
  • Reperfusion
  • Superior mesenteric artery

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Murine model of intestinal ischemia-reperfusion injury'. Together they form a unique fingerprint.

  • Cite this