Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates

M. A. Pfaller; Q. Vu; M. Lancaster; A. Espinel-Ingroff; A. Fothergill; C. Grant; M. R. McGinnis; L. Pasarell; M. G. Rinaldi; L. Steele-Moore

doi:10.1128/jcm.32.7.1625-1628.1994

Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates

M. A. Pfaller, Q. Vu, M. Lancaster, A. Espinel-Ingroff, A. Fothergill, C. Grant, M. R. McGinnis, L. Pasarell, M. G. Rinaldi, L. Steele-Moore

Pathology

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode ± 1 log₂ dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.65 with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.

Original language	English (US)
Pages (from-to)	1625-1628
Number of pages	4
Journal	Journal of clinical microbiology
Volume	32
Issue number	7
DOIs	https://doi.org/10.1128/jcm.32.7.1625-1628.1994
State	Published - 1994

ASJC Scopus subject areas

Microbiology (medical)

Access to Document

10.1128/jcm.32.7.1625-1628.1994

Fingerprint Dive into the research topics of 'Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates'. Together they form a unique fingerprint.

Cite this

Pfaller, M. A., Vu, Q., Lancaster, M., Espinel-Ingroff, A., Fothergill, A., Grant, C., McGinnis, M. R., Pasarell, L., Rinaldi, M. G., & Steele-Moore, L. (1994). Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates. Journal of clinical microbiology, 32(7), 1625-1628. https://doi.org/10.1128/jcm.32.7.1625-1628.1994

Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates. / Pfaller, M. A.; Vu, Q.; Lancaster, M.; Espinel-Ingroff, A.; Fothergill, A.; Grant, C.; McGinnis, M. R.; Pasarell, L.; Rinaldi, M. G.; Steele-Moore, L.

In: Journal of clinical microbiology, Vol. 32, No. 7, 1994, p. 1625-1628.

Research output: Contribution to journal › Article › peer-review

Pfaller, MA, Vu, Q, Lancaster, M, Espinel-Ingroff, A, Fothergill, A, Grant, C, McGinnis, MR, Pasarell, L, Rinaldi, MG & Steele-Moore, L 1994, 'Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates', Journal of clinical microbiology, vol. 32, no. 7, pp. 1625-1628. https://doi.org/10.1128/jcm.32.7.1625-1628.1994

@article{cb2599ad65d04a1a8e99812b88f33586,

title = "Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates",

abstract = "MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode ± 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.65 with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.",

author = "Pfaller, {M. A.} and Q. Vu and M. Lancaster and A. Espinel-Ingroff and A. Fothergill and C. Grant and McGinnis, {M. R.} and L. Pasarell and Rinaldi, {M. G.} and L. Steele-Moore",

year = "1994",

doi = "10.1128/jcm.32.7.1625-1628.1994",

language = "English (US)",

volume = "32",

pages = "1625--1628",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "7",

}

TY - JOUR

T1 - Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates

AU - Pfaller, M. A.

AU - Vu, Q.

AU - Lancaster, M.

AU - Espinel-Ingroff, A.

AU - Fothergill, A.

AU - Grant, C.

AU - McGinnis, M. R.

AU - Pasarell, L.

AU - Rinaldi, M. G.

AU - Steele-Moore, L.

PY - 1994

Y1 - 1994

N2 - MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode ± 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.65 with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.

AB - MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode ± 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.65 with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.

UR - http://www.scopus.com/inward/record.url?scp=0028343130&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028343130&partnerID=8YFLogxK

U2 - 10.1128/jcm.32.7.1625-1628.1994

DO - 10.1128/jcm.32.7.1625-1628.1994

M3 - Article

C2 - 7929747

AN - SCOPUS:0028343130

VL - 32

SP - 1625

EP - 1628

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 7

ER -