Abstract
MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode ± 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.65 with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1625-1628 |
| Number of pages | 4 |
| Journal | Journal of Clinical Microbiology |
| Volume | 32 |
| Issue number | 7 |
| State | Published - 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)
Cite this
Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates. / Pfaller, M. A.; Vu, Q.; Lancaster, M.; Espinel-Ingroff, A.; Fothergill, A.; Grant, C.; McGinnis, M. R.; Pasarell, L.; Rinaldi, M. G.; Steele-Moore, L.
In: Journal of Clinical Microbiology, Vol. 32, No. 7, 1994, p. 1625-1628.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates
AU - Pfaller, M. A.
AU - Vu, Q.
AU - Lancaster, M.
AU - Espinel-Ingroff, A.
AU - Fothergill, A.
AU - Grant, C.
AU - McGinnis, M. R.
AU - Pasarell, L.
AU - Rinaldi, M. G.
AU - Steele-Moore, L.
PY - 1994
Y1 - 1994
N2 - MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode ± 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.65 with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.
AB - MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode ± 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.65 with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.
UR - http://www.scopus.com/inward/record.url?scp=0028343130&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028343130&partnerID=8YFLogxK
M3 - Article
C2 - 7929747
AN - SCOPUS:0028343130
VL - 32
SP - 1625
EP - 1628
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 7
ER -