Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates

M. A. Pfaller, Q. Vu, M. Lancaster, A. Espinel-Ingroff, A. Fothergill, C. Grant, M. R. McGinnis, L. Pasarell, M. G. Rinaldi, L. Steele-Moore

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode ± 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.65 with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.

Original languageEnglish (US)
Pages (from-to)1625-1628
Number of pages4
JournalJournal of clinical microbiology
Volume32
Issue number7
DOIs
StatePublished - 1994

ASJC Scopus subject areas

  • Microbiology (medical)

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