Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates

M. A. Pfaller, Q. Vu, M. Lancaster, A. Espinel-Ingroff, A. Fothergill, C. Grant, M. R. McGinnis, L. Pasarell, M. G. Rinaldi, L. Steele-Moore

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode ± 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.65 with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.

Original languageEnglish (US)
Pages (from-to)1625-1628
Number of pages4
JournalJournal of Clinical Microbiology
Volume32
Issue number7
StatePublished - 1994
Externally publishedYes

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Fluconazole
Amphotericin B
Yeasts
Quality Control
Reproducibility of Results
Oxidation-Reduction

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Pfaller, M. A., Vu, Q., Lancaster, M., Espinel-Ingroff, A., Fothergill, A., Grant, C., ... Steele-Moore, L. (1994). Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates. Journal of Clinical Microbiology, 32(7), 1625-1628.

Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates. / Pfaller, M. A.; Vu, Q.; Lancaster, M.; Espinel-Ingroff, A.; Fothergill, A.; Grant, C.; McGinnis, M. R.; Pasarell, L.; Rinaldi, M. G.; Steele-Moore, L.

In: Journal of Clinical Microbiology, Vol. 32, No. 7, 1994, p. 1625-1628.

Research output: Contribution to journalArticle

Pfaller, MA, Vu, Q, Lancaster, M, Espinel-Ingroff, A, Fothergill, A, Grant, C, McGinnis, MR, Pasarell, L, Rinaldi, MG & Steele-Moore, L 1994, 'Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates', Journal of Clinical Microbiology, vol. 32, no. 7, pp. 1625-1628.
Pfaller MA, Vu Q, Lancaster M, Espinel-Ingroff A, Fothergill A, Grant C et al. Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates. Journal of Clinical Microbiology. 1994;32(7):1625-1628.
Pfaller, M. A. ; Vu, Q. ; Lancaster, M. ; Espinel-Ingroff, A. ; Fothergill, A. ; Grant, C. ; McGinnis, M. R. ; Pasarell, L. ; Rinaldi, M. G. ; Steele-Moore, L. / Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates. In: Journal of Clinical Microbiology. 1994 ; Vol. 32, No. 7. pp. 1625-1628.
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