Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates

M. A. Pfaller; Q. Vu; M. Lancaster; A. Espinel-Ingroff; A. Fothergill; C. Grant; M. R. McGinnis; L. Pasarell; M. G. Rinaldi; L. Steele-Moore

doi:10.1128/jcm.32.7.1625-1628.1994

Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates

M. A. Pfaller, Q. Vu, M. Lancaster, A. Espinel-Ingroff, A. Fothergill, C. Grant, M. R. McGinnis, L. Pasarell, M. G. Rinaldi, L. Steele-Moore

Department of Pathology

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode ± 1 log₂ dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.65 with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.

Original language	English (US)
Pages (from-to)	1625-1628
Number of pages	4
Journal	Journal of clinical microbiology
Volume	32
Issue number	7
DOIs	https://doi.org/10.1128/jcm.32.7.1625-1628.1994
State	Published - 1994

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/jcm.32.7.1625-1628.1994

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Pfaller, M. A., Vu, Q., Lancaster, M., Espinel-Ingroff, A., Fothergill, A., Grant, C., McGinnis, M. R., Pasarell, L., Rinaldi, M. G., & Steele-Moore, L. (1994). Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates. Journal of clinical microbiology, 32(7), 1625-1628. https://doi.org/10.1128/jcm.32.7.1625-1628.1994

Pfaller, MA, Vu, Q, Lancaster, M, Espinel-Ingroff, A, Fothergill, A, Grant, C, McGinnis, MR, Pasarell, L, Rinaldi, MG & Steele-Moore, L 1994, 'Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates', Journal of clinical microbiology, vol. 32, no. 7, pp. 1625-1628. https://doi.org/10.1128/jcm.32.7.1625-1628.1994

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AU - Pfaller, M. A.

AU - Vu, Q.

AU - Lancaster, M.

AU - Espinel-Ingroff, A.

AU - Fothergill, A.

AU - Grant, C.

AU - McGinnis, M. R.

AU - Pasarell, L.

AU - Rinaldi, M. G.

AU - Steele-Moore, L.

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AB - MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode ± 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.65 with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.

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Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates

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