Multiplexed targeted mass spectrometry assays for prostate cancer-associated urinary proteins

Tujin Shi, Sue Ing Quek, Yuqian Gao, Carrie D. Nicora, Song Nie, Thomas L. Fillmore, Tao Liu, Karin D. Rodland, Richard D. Smith, Robin J. Leach, Ian M. Thompson, Elizabeth A. Vitello, William J. Ellis, Alvin Y. Liu, Wei Jun Qian

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Biomarkers for effective early diagnosis and prognosis of prostate cancer are still lacking. Multiplexed assays for cancer-associated proteins could be useful for identifying biomarkers for cancer detection and stratification. Herein, we report the development of sensitive targeted mass spectrometry assays for simultaneous quantification of 10 prostate cancer-associated proteins in urine. The diagnostic utility of these markers was evaluated with an initial cohort of 20 clinical urine samples. Individual marker concentration was normalized against the measured urinary prostate-specific antigen level as a reference of prostate-specific secretion. The areas under the receiver-operating characteristic curves for the 10 proteins ranged from 0.75 for CXL14 to 0.87 for CEAM5. Furthermore, MMP9 level was found to be significantly higher in patients with high Gleason scores, suggesting a potential of MMP9 as a marker for risk level assessment. Taken together, our work illustrated the feasibility of accurate multiplexed measurements of low-abundance cancer-associated proteins in urine and provided a viable path forward for preclinical verification of candidate biomarkers for prostate cancer.

Original languageEnglish (US)
Pages (from-to)101887-101898
Number of pages12
JournalOncotarget
Volume8
Issue number60
DOIs
StatePublished - 2017

Keywords

  • Prostate cancer
  • Prostate cancer detection
  • Secreted protein biomarkers
  • Selected reaction monitoring
  • Targeted mass spectrometry

ASJC Scopus subject areas

  • Oncology

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  • Cite this

    Shi, T., Quek, S. I., Gao, Y., Nicora, C. D., Nie, S., Fillmore, T. L., Liu, T., Rodland, K. D., Smith, R. D., Leach, R. J., Thompson, I. M., Vitello, E. A., Ellis, W. J., Liu, A. Y., & Qian, W. J. (2017). Multiplexed targeted mass spectrometry assays for prostate cancer-associated urinary proteins. Oncotarget, 8(60), 101887-101898. https://doi.org/10.18632/oncotarget.21710