Multiple Purkinje cell recording in rodent cerebellar cortex

K. Sasaki, J. M. Bower, R. Llinas

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

The spatial and temporal organization of climbing fibre activation of Purkinje cells, the so-called complex spikes, were studied in the rat cerebellar Crus II folium utilizing a multiple microelectrode recording technique. As many as 32 Purkinje cells could be simultaneously recorded by using a custom-built electronic amplifier system and a special data storage device. Analysis of the auto-correlation activity of complex spikes in any given group of Purkinje cells indicated that activation occurs with a particular rhythmicity having a base firing of 10 Hz. Cross-correlation of spontaneous complex spikes demonstrated, in addition to a particular rhythmicity, an extraordinarily high degree of synchronicity within a particular spatial distribution of Purkinje cells. Thus, Purkinje cells organized in rostro-caudal rows tend to fire within 1 ms of each other for distances as far as 800 μm (the width of a folium) from the 'master' neuron. By contrast, Purkinje cells located medial or lateral to the master neuron showed almost no cross-correlation. Administration of harmaline to the animal increased the degree of auto- and cross-correlation but did not change the spatial order of the distribution of the cross-correlation. The results indicate that the olivo-cerebellar system is organized in such a way that climbing fibre afferents may be activated in a close-to-synchronous and rhythmic fashion. The spatial distribution of these afferents over the cortex is such as to activate rostro-caudal bands of Purkinje cells which tend to fire in a close-to-synchronous manner.

Original languageEnglish (US)
Pages (from-to)572-586
Number of pages15
JournalEuropean Journal of Neuroscience
Volume1
Issue number6
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Cerebellar Cortex
Purkinje Cells
Rodentia
Periodicity
Electronic Amplifiers
Harmaline
Neurons
Information Storage and Retrieval
Microelectrodes
Equipment and Supplies

Keywords

  • Autorhythmicity
  • Complex spikes
  • Motor coordination
  • Oscillations
  • Timing

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Multiple Purkinje cell recording in rodent cerebellar cortex. / Sasaki, K.; Bower, J. M.; Llinas, R.

In: European Journal of Neuroscience, Vol. 1, No. 6, 1989, p. 572-586.

Research output: Contribution to journalArticle

Sasaki, K. ; Bower, J. M. ; Llinas, R. / Multiple Purkinje cell recording in rodent cerebellar cortex. In: European Journal of Neuroscience. 1989 ; Vol. 1, No. 6. pp. 572-586.
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