Multiple mechanisms inactivate the LIN-41 RNA-binding protein to ensure a robust oocyte-to-embryo transition in caenorhabditis elegans

Caroline A. Spike, Gabriela Huelgas-Morales, Tatsuya Tsukamoto, David Greenstein

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

In the nematode Caenorhabditis elegans, the conserved LIN-41 RNA-binding protein is a translational repressor that coordinately controls oocyte growth and meiotic maturation. LIN-41 exerts these effects, at least in part, by preventing the premature activation of the cyclin-dependent kinase CDK-1. Here we investigate the mechanism by which LIN-41 is rapidly eliminated upon the onset of meiotic maturation. Elimination of LIN-41 requires the activities of CDK-1 and multiple SCF (Skp1, Cul1, and F-box protein)-type E3 ubiquitin ligase subunits, including the conserved substrate adaptor protein SEL-10/Fbw7/Cdc4, suggesting that LIN-41 is a target of ubiquitin-mediated protein degradation. Within the LIN-41 protein, two nonoverlapping regions, Deg-A and Deg-B, are individually necessary for LIN-41 degradation; both contain several potential phosphodegron sequences, and at least one of these sequences is required for LIN-41 degradation. Finally, Deg-A and Deg-B are sufficient, in combination, to mediate SEL-10-dependent degradation when transplanted into a different oocyte protein. Although LIN-41 is a potent inhibitor of protein translation and M phase entry, the failure to eliminate LIN-41 from early embryos does not result in the continued translational repression of LIN-41 oocyte messenger RNA targets. Based on these observations, we propose a model for the elimination of LIN-41 by the SEL-10 E3 ubiquitin ligase and suggest that LIN-41 is inactivated before it is degraded. Furthermore, we provide evidence that another RNA-binding protein, the GLD-1 tumor suppressor, is regulated similarly. Redundant mechanisms to extinguish translational repression by RNA-binding proteins may both control and provide robustness to irreversible developmental transitions, including meiotic maturation and the oocyte-to-embryo transition.

Original languageEnglish (US)
Pages (from-to)1011-1037
Number of pages27
JournalGenetics
Volume210
Issue number3
DOIs
StatePublished - Nov 2018
Externally publishedYes

Keywords

  • Oocyte meiotic maturation
  • Oocyte-to-embryo transition
  • RNA-binding proteins
  • Translational regulation
  • Ubiquitin-mediated protein degradation

ASJC Scopus subject areas

  • Genetics

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