Multiparametric and longitudinal MRI characterization of mild traumatic brain injury in rats

Justin Alexander Long, Lora Talley Watts, Jonathan Chemello, Shiliang Huang, Qiang Shen, Timothy Q. Duong

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

This study reports T2 and diffusion-tensor magnetic resonance imaging (MRI) studies of a mild open-skull, controlled cortical impact injury in rats (n=6) from 3 h to up to 14 d after traumatic brain injury (TBI). Comparison was made with longitudinal behavioral measurements and end-point histology. The impact was applied over the left primary forelimb somatosensory cortex (S1FL). The major findings were: 1) In the S1FL, T2 increased and fractional anisotropy (FA) decreased at 3 h after TBI and gradually returned toward normal by Day 14; 2) in the S1FL, the apparent diffusion coefficient (ADC) increased at 3 h, peaked on Day 2, and gradually returned toward normal at Day 14; 3) in the corpus callosum underneath the S1FL, FA decreased at 3 h to Day 2 but returned to normal at Day 7 and 14, whereas T2 and ADC were normal throughout; 4) heterogeneous hyperintense and hypointense T2 map intensities likely indicated the presence of hemorrhage but were not independently verified; 5) lesion volumes defined by abnormal T2, ADC, and FA showed similar temporal patterns, peaking around Day 2 and returning toward normal on Day 14; 6) the temporal profiles of lesion volumes were consistent with behavioral scores assessed by forelimb placement and forelimb foot fault tests; and 7) at 14 d post-TBI, there was substantial tissue recovery by MRI, which could either reflect true tissue recovery or reabsorption of edema. Histology performed 14 d post-TBI, however, showed a small cavitation and significant neuronal degeneration surrounding the cavitation in S1FL. Thus, the observed improvement of behavioral scores likely involves both functional recovery and functional compensation.

Original languageEnglish (US)
Pages (from-to)598-607
Number of pages10
JournalJournal of Neurotrauma
Volume32
Issue number8
DOIs
StatePublished - Apr 15 2015

Fingerprint

Brain Concussion
Forelimb
Anisotropy
Magnetic Resonance Imaging
Histology
Diffusion Magnetic Resonance Imaging
Somatosensory Cortex
Corpus Callosum
Skull
Foot
Edema
Hemorrhage
Traumatic Brain Injury
Wounds and Injuries

Keywords

  • behavioral assessments
  • controlled cortical impact
  • diffusion tensor imaging
  • immunohistochemistry
  • quantitative magnetic resonance imaging
  • TBI

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Long, J. A., Watts, L. T., Chemello, J., Huang, S., Shen, Q., & Duong, T. Q. (2015). Multiparametric and longitudinal MRI characterization of mild traumatic brain injury in rats. Journal of Neurotrauma, 32(8), 598-607. https://doi.org/10.1089/neu.2014.3563

Multiparametric and longitudinal MRI characterization of mild traumatic brain injury in rats. / Long, Justin Alexander; Watts, Lora Talley; Chemello, Jonathan; Huang, Shiliang; Shen, Qiang; Duong, Timothy Q.

In: Journal of Neurotrauma, Vol. 32, No. 8, 15.04.2015, p. 598-607.

Research output: Contribution to journalArticle

Long, JA, Watts, LT, Chemello, J, Huang, S, Shen, Q & Duong, TQ 2015, 'Multiparametric and longitudinal MRI characterization of mild traumatic brain injury in rats', Journal of Neurotrauma, vol. 32, no. 8, pp. 598-607. https://doi.org/10.1089/neu.2014.3563
Long, Justin Alexander ; Watts, Lora Talley ; Chemello, Jonathan ; Huang, Shiliang ; Shen, Qiang ; Duong, Timothy Q. / Multiparametric and longitudinal MRI characterization of mild traumatic brain injury in rats. In: Journal of Neurotrauma. 2015 ; Vol. 32, No. 8. pp. 598-607.
@article{0fbf43597f17497d8216d634d2b71dd1,
title = "Multiparametric and longitudinal MRI characterization of mild traumatic brain injury in rats",
abstract = "This study reports T2 and diffusion-tensor magnetic resonance imaging (MRI) studies of a mild open-skull, controlled cortical impact injury in rats (n=6) from 3 h to up to 14 d after traumatic brain injury (TBI). Comparison was made with longitudinal behavioral measurements and end-point histology. The impact was applied over the left primary forelimb somatosensory cortex (S1FL). The major findings were: 1) In the S1FL, T2 increased and fractional anisotropy (FA) decreased at 3 h after TBI and gradually returned toward normal by Day 14; 2) in the S1FL, the apparent diffusion coefficient (ADC) increased at 3 h, peaked on Day 2, and gradually returned toward normal at Day 14; 3) in the corpus callosum underneath the S1FL, FA decreased at 3 h to Day 2 but returned to normal at Day 7 and 14, whereas T2 and ADC were normal throughout; 4) heterogeneous hyperintense and hypointense T2 map intensities likely indicated the presence of hemorrhage but were not independently verified; 5) lesion volumes defined by abnormal T2, ADC, and FA showed similar temporal patterns, peaking around Day 2 and returning toward normal on Day 14; 6) the temporal profiles of lesion volumes were consistent with behavioral scores assessed by forelimb placement and forelimb foot fault tests; and 7) at 14 d post-TBI, there was substantial tissue recovery by MRI, which could either reflect true tissue recovery or reabsorption of edema. Histology performed 14 d post-TBI, however, showed a small cavitation and significant neuronal degeneration surrounding the cavitation in S1FL. Thus, the observed improvement of behavioral scores likely involves both functional recovery and functional compensation.",
keywords = "behavioral assessments, controlled cortical impact, diffusion tensor imaging, immunohistochemistry, quantitative magnetic resonance imaging, TBI",
author = "Long, {Justin Alexander} and Watts, {Lora Talley} and Jonathan Chemello and Shiliang Huang and Qiang Shen and Duong, {Timothy Q.}",
year = "2015",
month = "4",
day = "15",
doi = "10.1089/neu.2014.3563",
language = "English (US)",
volume = "32",
pages = "598--607",
journal = "Journal of Neurotrauma",
issn = "0897-7151",
publisher = "Mary Ann Liebert Inc.",
number = "8",

}

TY - JOUR

T1 - Multiparametric and longitudinal MRI characterization of mild traumatic brain injury in rats

AU - Long, Justin Alexander

AU - Watts, Lora Talley

AU - Chemello, Jonathan

AU - Huang, Shiliang

AU - Shen, Qiang

AU - Duong, Timothy Q.

PY - 2015/4/15

Y1 - 2015/4/15

N2 - This study reports T2 and diffusion-tensor magnetic resonance imaging (MRI) studies of a mild open-skull, controlled cortical impact injury in rats (n=6) from 3 h to up to 14 d after traumatic brain injury (TBI). Comparison was made with longitudinal behavioral measurements and end-point histology. The impact was applied over the left primary forelimb somatosensory cortex (S1FL). The major findings were: 1) In the S1FL, T2 increased and fractional anisotropy (FA) decreased at 3 h after TBI and gradually returned toward normal by Day 14; 2) in the S1FL, the apparent diffusion coefficient (ADC) increased at 3 h, peaked on Day 2, and gradually returned toward normal at Day 14; 3) in the corpus callosum underneath the S1FL, FA decreased at 3 h to Day 2 but returned to normal at Day 7 and 14, whereas T2 and ADC were normal throughout; 4) heterogeneous hyperintense and hypointense T2 map intensities likely indicated the presence of hemorrhage but were not independently verified; 5) lesion volumes defined by abnormal T2, ADC, and FA showed similar temporal patterns, peaking around Day 2 and returning toward normal on Day 14; 6) the temporal profiles of lesion volumes were consistent with behavioral scores assessed by forelimb placement and forelimb foot fault tests; and 7) at 14 d post-TBI, there was substantial tissue recovery by MRI, which could either reflect true tissue recovery or reabsorption of edema. Histology performed 14 d post-TBI, however, showed a small cavitation and significant neuronal degeneration surrounding the cavitation in S1FL. Thus, the observed improvement of behavioral scores likely involves both functional recovery and functional compensation.

AB - This study reports T2 and diffusion-tensor magnetic resonance imaging (MRI) studies of a mild open-skull, controlled cortical impact injury in rats (n=6) from 3 h to up to 14 d after traumatic brain injury (TBI). Comparison was made with longitudinal behavioral measurements and end-point histology. The impact was applied over the left primary forelimb somatosensory cortex (S1FL). The major findings were: 1) In the S1FL, T2 increased and fractional anisotropy (FA) decreased at 3 h after TBI and gradually returned toward normal by Day 14; 2) in the S1FL, the apparent diffusion coefficient (ADC) increased at 3 h, peaked on Day 2, and gradually returned toward normal at Day 14; 3) in the corpus callosum underneath the S1FL, FA decreased at 3 h to Day 2 but returned to normal at Day 7 and 14, whereas T2 and ADC were normal throughout; 4) heterogeneous hyperintense and hypointense T2 map intensities likely indicated the presence of hemorrhage but were not independently verified; 5) lesion volumes defined by abnormal T2, ADC, and FA showed similar temporal patterns, peaking around Day 2 and returning toward normal on Day 14; 6) the temporal profiles of lesion volumes were consistent with behavioral scores assessed by forelimb placement and forelimb foot fault tests; and 7) at 14 d post-TBI, there was substantial tissue recovery by MRI, which could either reflect true tissue recovery or reabsorption of edema. Histology performed 14 d post-TBI, however, showed a small cavitation and significant neuronal degeneration surrounding the cavitation in S1FL. Thus, the observed improvement of behavioral scores likely involves both functional recovery and functional compensation.

KW - behavioral assessments

KW - controlled cortical impact

KW - diffusion tensor imaging

KW - immunohistochemistry

KW - quantitative magnetic resonance imaging

KW - TBI

UR - http://www.scopus.com/inward/record.url?scp=84926654632&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84926654632&partnerID=8YFLogxK

U2 - 10.1089/neu.2014.3563

DO - 10.1089/neu.2014.3563

M3 - Article

C2 - 25203249

AN - SCOPUS:84926654632

VL - 32

SP - 598

EP - 607

JO - Journal of Neurotrauma

JF - Journal of Neurotrauma

SN - 0897-7151

IS - 8

ER -