TY - JOUR
T1 - Multidimensional Approach Assessing the Role of Interleukin 1 Beta in Mesial Temporal Lobe Epilepsy
AU - Santos, Renato O.
AU - Secolin, Rodrigo
AU - Barbalho, Patrícia G.
AU - Silva-Alves, Mariana S.
AU - Alvim, Marina K.M.
AU - Yasuda, Clarissa L.
AU - Rogerio, Fábio
AU - Velasco, Tonicarlo R.
AU - Sakamoto, Americo C.
AU - Teixeira, Antonio L.
AU - Cendes, Fernando
AU - Maurer-Morelli, Claudia V.
AU - Lopes-Cendes, Iscia
N1 - Publisher Copyright:
© Copyright © 2021 Santos, Secolin, Barbalho, Silva-Alves, Alvim, Yasuda, Rogerio, Velasco, Sakamoto, Teixeira, Cendes, Maurer-Morelli and Lopes-Cendes.
PY - 2021/8/5
Y1 - 2021/8/5
N2 - We aimed to investigate the role of interleukin-1 beta (IL-1β) in the mechanisms underlying mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE+HS). We assessed a cohort of 194 patients with MTLE+HS and 199 healthy controls. Patients were divided into those with positive and negative antecedent febrile seizures (FS). We used a multidimensional approach, including (i) genetic association with single nucleotide polymorphisms (SNPs) in the IL1B gene; (ii) quantification of the IL1B transcript in the hippocampal tissue of patients with refractory seizures; and (iii) quantification of the IL-1β protein in the plasma. We found a genetic association signal for two SNPs, rs2708928 and rs3730364*C in the IL1B gene, regardless of the presence of FS (adjusted p = 9.62e–11 and 5.14e–07, respectively). We found no difference between IL1B transcript levels when comparing sclerotic hippocampal tissue from patients with MTLE+HS, without FS, and hippocampi from autopsy controls (p > 0.05). Nevertheless, we found increased IL-1β in the plasma of patients with MTLE+HS with FS compared with controls (p = 0.0195). Our results support the hypothesis of a genetic association between MTLE+HS and the IL1B gene.
AB - We aimed to investigate the role of interleukin-1 beta (IL-1β) in the mechanisms underlying mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE+HS). We assessed a cohort of 194 patients with MTLE+HS and 199 healthy controls. Patients were divided into those with positive and negative antecedent febrile seizures (FS). We used a multidimensional approach, including (i) genetic association with single nucleotide polymorphisms (SNPs) in the IL1B gene; (ii) quantification of the IL1B transcript in the hippocampal tissue of patients with refractory seizures; and (iii) quantification of the IL-1β protein in the plasma. We found a genetic association signal for two SNPs, rs2708928 and rs3730364*C in the IL1B gene, regardless of the presence of FS (adjusted p = 9.62e–11 and 5.14e–07, respectively). We found no difference between IL1B transcript levels when comparing sclerotic hippocampal tissue from patients with MTLE+HS, without FS, and hippocampi from autopsy controls (p > 0.05). Nevertheless, we found increased IL-1β in the plasma of patients with MTLE+HS with FS compared with controls (p = 0.0195). Our results support the hypothesis of a genetic association between MTLE+HS and the IL1B gene.
KW - association study
KW - gene expression
KW - hippocampal atrophy
KW - mesial temporal sclerosis
KW - neuroinflammation
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U2 - 10.3389/fneur.2021.690847
DO - 10.3389/fneur.2021.690847
M3 - Article
AN - SCOPUS:85113232029
SN - 1664-2295
VL - 12
JO - Frontiers in Neurology
JF - Frontiers in Neurology
M1 - 690847
ER -