Multicenter evaluation of proposed standardized procedure for antifungal susceptibility testing of filamentous fungi

A. Espinel-Ingroff, M. Bartlett, R. Bowden, N. X. Chin, C. Cooper, A. Fothergill, M. R. McGinnis, P. Menezes, S. A. Messer, P. W. Nelson, F. C. Odds, L. Pasarell, J. Peter, M. A. Pfaller, J. H. Rex, M. G. Rinaldi, G. S. Shankland, T. J. Walsh, I. Weitzman

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213 Scopus citations

Abstract

A multicenter study was conducted to expand the generation and analysis of data that supports the proposal of a reference method for the antifungal susceptibility testing of filamentous fungi. Broth microdilution MICs of amphotericin B and itraconazole were determined in 11 centers against 30 coded duplicate pairs of Aspergillus spp., Fusarium spp., Pseudallescheria boydii, and Rhizopus arrhizus. The effect of inoculum density (approximately 103 and 104 CFU/ml), incubation time (24, 48, and 72 h), and procedure of MIC determination (conventional and colorimetric [Alamar Blue] evaluation of growth inhibition) on intra- and interlaboratory agreement was analyzed. Based on intra- (97 to 100%) and interlaboratory (94 to 95%) agreement for both drugs, the overall optimal testing conditions identified were determination of colorimetric MICs after 48 to 72 h of incubation with an inoculum density of approximately 104 CFU/ml. These testing conditions are proposed as guidelines for a reference broth microdilution method.

Original languageEnglish (US)
Pages (from-to)139-143
Number of pages5
JournalJournal of clinical microbiology
Volume35
Issue number1
DOIs
StatePublished - Jan 1997

ASJC Scopus subject areas

  • Microbiology (medical)

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    Espinel-Ingroff, A., Bartlett, M., Bowden, R., Chin, N. X., Cooper, C., Fothergill, A., McGinnis, M. R., Menezes, P., Messer, S. A., Nelson, P. W., Odds, F. C., Pasarell, L., Peter, J., Pfaller, M. A., Rex, J. H., Rinaldi, M. G., Shankland, G. S., Walsh, T. J., & Weitzman, I. (1997). Multicenter evaluation of proposed standardized procedure for antifungal susceptibility testing of filamentous fungi. Journal of clinical microbiology, 35(1), 139-143. https://doi.org/10.1128/jcm.35.1.139-143.1997