Multicenter comparison of a colorimetric microdilution broth method with the reference macrodilution method for in vitro susceptibility testing of yeast isolates

Michael A. Pfaller, Barry Buschelman, Martha J. Bale, Michael Lancaster, Ana Espinel-Ingroff, John H. Rex, Michael G. Rinaldi

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

This multicenter study was performed to compare a colorimetric microdilution method with the NCCLS M27-P reference macrodilution method for the testing of yeast isolates against amphotericin B, fluconazole, and 5-fluorocytosine (5FC). Testing was performed on ten yeast isolates in five independent laboratories. All sites tested each isolate a total of 20 times with both methods. MICs were read after 48 h incubation. The macrodilution MIC reference range was defined as the modal MIC ± 1-log2 dilution for each organism-antifungal agent combination. Agreement between the M27-P reference range results and the microdilution MICs was 86% with amphotericin B, 90% with fluconazole, and 93% with 5FC. Based on these data, it is apparent that new approaches, such as the colorimetric microdilution method, will provide MIC values comparable to the M27-P macrodilution method in a format that is more practical for use in a busy clinical laboratory.

Original languageEnglish (US)
Pages (from-to)9-13
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume19
Issue number1
DOIs
StatePublished - May 1994
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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