Multi-dimensional mass spectrometry-based shotgun lipidomics and novel strategies for lipidomic analyses

Xianlin Han, Kui Yang, Richard W. Gross

Research output: Contribution to journalReview articlepeer-review

420 Scopus citations


Since our last comprehensive review on multi-dimensional mass spectrometry-based shotgun lipidomics (Mass Spectrom. Rev. 24 (2005), 367), many new developments in the field of lipidomics have occurred. These developments include new strategies and refinements for shotgun lipidomic approaches that use direct infusion, including novel fragmentation strategies, identification of multiple new informative dimensions for mass spectrometric interrogation, and the development of new bioinformatic approaches for enhanced identification and quantitation of the individual molecular constituents that comprise each cell's lipidome. Concurrently, advances in liquid chromatography-based platforms and novel strategies for quantitative matrix-assisted laser desorption/ionization mass spectrometry for lipidomic analyses have been developed. Through the synergistic use of this repertoire of new mass spectrometric approaches, the power and scope of lipidomics has been greatly expanded to accelerate progress toward the comprehensive understanding of the pleiotropic roles of lipids in biological systems.

Original languageEnglish (US)
Pages (from-to)134-178
Number of pages45
JournalMass Spectrometry Reviews
Issue number1
StatePublished - Jan 2012
Externally publishedYes


  • electrospray ionization
  • lipidomics
  • mass spectrometry
  • shotgun lipidomics

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Analytical Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Spectroscopy


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