mTORC1 underlies age-related muscle fiber damage and loss by inducing oxidative stress and catabolism

Huibin Tang, Ken Inoki, Susan V. Brooks, Hideki Okazawa, Myung Lee, Junying Wang, Michael Kim, Catherine L. Kennedy, Peter C.D. Macpherson, Xuhuai Ji, Sabrina Van Roekel, Danielle A. Fraga, Kun Wang, Jinguo Zhu, Yoyo Wang, Zelton D Sharp, Richard A. Miller, Thomas A. Rando, Daniel Goldman, Kun Liang GuanJoseph B. Shrager

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aging leads to skeletal muscle atrophy (i.e., sarcopenia), and muscle fiber loss is a critical component of this process. The mechanisms underlying these age-related changes, however, remain unclear. We show here that mTORC1 signaling is activated in a subset of skeletal muscle fibers in aging mouse and human, colocalized with fiber damage. Activation of mTORC1 in TSC1 knockout mouse muscle fibers increases the content of morphologically abnormal mitochondria and causes progressive oxidative stress, fiber damage, and fiber loss over the lifespan. Transcriptomic profiling reveals that mTORC1's activation increases the expression of growth differentiation factors (GDF3, 5, and 15), and of genes involved in mitochondrial oxidative stress and catabolism. We show that increased GDF15 is sufficient to induce oxidative stress and catabolic changes, and that mTORC1 increases the expression of GDF15 via phosphorylation of STAT3. Inhibition of mTORC1 in aging mouse decreases the expression of GDFs and STAT3's phosphorylation in skeletal muscle, reducing oxidative stress and muscle fiber damage and loss. Thus, chronically increased mTORC1 activity contributes to age-related muscle atrophy, and GDF signaling is a proposed mechanism.

Original languageEnglish (US)
Article numbere12943
JournalAging Cell
Volume18
Issue number3
DOIs
StatePublished - Jun 1 2019

Fingerprint

Oxidative Stress
Muscles
Stress Fibers
Muscular Atrophy
Skeletal Muscle
Growth Differentiation Factor 5
Growth Differentiation Factor 15
Phosphorylation
Sarcopenia
Skeletal Muscle Fibers
Knockout Mice
mechanistic target of rapamycin complex 1
Mitochondria
Genes

Keywords

  • aging
  • mTORC1
  • oxidative stress
  • signal transduction
  • skeletal muscle

ASJC Scopus subject areas

  • Aging
  • Cell Biology

Cite this

Tang, H., Inoki, K., Brooks, S. V., Okazawa, H., Lee, M., Wang, J., ... Shrager, J. B. (2019). mTORC1 underlies age-related muscle fiber damage and loss by inducing oxidative stress and catabolism. Aging Cell, 18(3), [e12943]. https://doi.org/10.1111/acel.12943

mTORC1 underlies age-related muscle fiber damage and loss by inducing oxidative stress and catabolism. / Tang, Huibin; Inoki, Ken; Brooks, Susan V.; Okazawa, Hideki; Lee, Myung; Wang, Junying; Kim, Michael; Kennedy, Catherine L.; Macpherson, Peter C.D.; Ji, Xuhuai; Van Roekel, Sabrina; Fraga, Danielle A.; Wang, Kun; Zhu, Jinguo; Wang, Yoyo; Sharp, Zelton D; Miller, Richard A.; Rando, Thomas A.; Goldman, Daniel; Guan, Kun Liang; Shrager, Joseph B.

In: Aging Cell, Vol. 18, No. 3, e12943, 01.06.2019.

Research output: Contribution to journalArticle

Tang, H, Inoki, K, Brooks, SV, Okazawa, H, Lee, M, Wang, J, Kim, M, Kennedy, CL, Macpherson, PCD, Ji, X, Van Roekel, S, Fraga, DA, Wang, K, Zhu, J, Wang, Y, Sharp, ZD, Miller, RA, Rando, TA, Goldman, D, Guan, KL & Shrager, JB 2019, 'mTORC1 underlies age-related muscle fiber damage and loss by inducing oxidative stress and catabolism', Aging Cell, vol. 18, no. 3, e12943. https://doi.org/10.1111/acel.12943
Tang, Huibin ; Inoki, Ken ; Brooks, Susan V. ; Okazawa, Hideki ; Lee, Myung ; Wang, Junying ; Kim, Michael ; Kennedy, Catherine L. ; Macpherson, Peter C.D. ; Ji, Xuhuai ; Van Roekel, Sabrina ; Fraga, Danielle A. ; Wang, Kun ; Zhu, Jinguo ; Wang, Yoyo ; Sharp, Zelton D ; Miller, Richard A. ; Rando, Thomas A. ; Goldman, Daniel ; Guan, Kun Liang ; Shrager, Joseph B. / mTORC1 underlies age-related muscle fiber damage and loss by inducing oxidative stress and catabolism. In: Aging Cell. 2019 ; Vol. 18, No. 3.
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