mRNA transcription determines the lag period for the induction of pineal melatonin synthesis in the Syrian hamster pineal gland

A. Gonzalez-Brito, M. E. Troiani, A. Menendez-Pelaez, M. J. Delgado, Russel J Reiter

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The nocturnal pattern of Syrian hamster pineal melatonin synthesis is characterized by a 6-8 h lag period, followed by a late-night, short-duration peak in both N-acetyltransferase (NAT) activity and melatonin content. Administration of cycloheximide (20 mg/kg body weight) given either at the time of lights out or 4 h into the dark phase to Syrian hamsters blocked the nocturnal increase in both pineal NAT activity and melatonin content. Actinomycin D (5 mg/kg body weight) prevented the nocturnal increase in both constituents only when it was administered at darkness onset, being significantly less effective when injected after 4 h of dark exposure. Reinduction of melatonin production by isoproterenol (2 mg/kg body weight) administration to acutely light-exposed animals during late darkness was prevented by cycloheximide, but not by actinomycin D administration. The results suggest that whereas Syrian hamster pineal melatonin production requires protein synthesis both early and late in the dark phase, the transcription of a putative NAT-related mRNA, which occurs only during the early night, seems to determine the lag period in melatonin synthesis and pineal responsiveness to β-adrenergic receptor agonist stimulation.

Original languageEnglish (US)
Pages (from-to)55-60
Number of pages6
JournalJournal of Cellular Biochemistry
Volume44
Issue number1
StatePublished - 1990

Fingerprint

Pineal Gland
Mesocricetus
Melatonin
Transcription
Messenger RNA
Acetyltransferases
Darkness
Body Weight
Dactinomycin
Cycloheximide
Light
Adrenergic Agonists
Isoproterenol
Animals
Proteins

Keywords

  • β-adrenergic receptors
  • actinomycin D
  • cycloheximide
  • isoproterenol
  • nocturnal melatonin production

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

mRNA transcription determines the lag period for the induction of pineal melatonin synthesis in the Syrian hamster pineal gland. / Gonzalez-Brito, A.; Troiani, M. E.; Menendez-Pelaez, A.; Delgado, M. J.; Reiter, Russel J.

In: Journal of Cellular Biochemistry, Vol. 44, No. 1, 1990, p. 55-60.

Research output: Contribution to journalArticle

Gonzalez-Brito, A. ; Troiani, M. E. ; Menendez-Pelaez, A. ; Delgado, M. J. ; Reiter, Russel J. / mRNA transcription determines the lag period for the induction of pineal melatonin synthesis in the Syrian hamster pineal gland. In: Journal of Cellular Biochemistry. 1990 ; Vol. 44, No. 1. pp. 55-60.
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