Mouse platelet-derived growth factor receptor α gene is deleted in W(19H) and patch mutations on chromosome 5

E. A. Smith, M. F. Seldin, L. Martinez, M. L. Watson, G. G. Choudhury, P. A. Lalley, J. Pierce, S. Aaronson, J. Barker, S. L. Naylor, A. Y. Sakaguchi

Research output: Contribution to journalArticle

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Abstract

The mouse W(19H) mutation is an x-ray-induced deletion of more than 2 centimorgans on chromosome 5 encompassing the white spotting mutation W (encoded by the Kit protooncogene), patch (Ph), and recessive lethal (l) loci. The platelet-derived growth factor receptor α gene (PDGFRA) like Kit encodes a transmembrane receptor tyrosine kinase. By using mouse-Chinese hamster somatic cell hybrids and haplotype analysis in interspecific backcross mice, mouse Pdgfra was mapped to chromosome 5 in tight linkage with Kit. Hybridization of a PDGFRA probe to DNAs from W(19H)/+ heterozygous mice and patch heterozygous mice, and their wild-type littermates, demonstrated deletion of Pdgfra. Pulsed-field gel electrophoresis indicated that Kit and Pdgfra are linked on a 630-kilobase Mlu I DNA fragment. Thus the W(19H) deletion removes at least two receptor tyrosine kinases and the results suggest Pdgfra as a candidate for the Ph locus.

Original languageEnglish (US)
Pages (from-to)4811-4815
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number11
DOIs
StatePublished - Jan 1 1991

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