Mouse platelet-derived growth factor receptor α gene is deleted in W(19H) and patch mutations on chromosome 5

E. A. Smith; M. F. Seldin; L. Martinez; M. L. Watson; G. G. Choudhury; P. A. Lalley; J. Pierce; S. Aaronson; J. Barker; S. L. Naylor; A. Y. Sakaguchi

doi:10.1073/pnas.88.11.4811

Mouse platelet-derived growth factor receptor α gene is deleted in W(19H) and patch mutations on chromosome 5

E. A. Smith, M. F. Seldin, L. Martinez, M. L. Watson, G. G. Choudhury, P. A. Lalley, J. Pierce, S. Aaronson, J. Barker, S. L. Naylor, A. Y. Sakaguchi

Research output: Contribution to journal › Article

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Abstract

The mouse W(19H) mutation is an x-ray-induced deletion of more than 2 centimorgans on chromosome 5 encompassing the white spotting mutation W (encoded by the Kit protooncogene), patch (Ph), and recessive lethal (l) loci. The platelet-derived growth factor receptor α gene (PDGFRA) like Kit encodes a transmembrane receptor tyrosine kinase. By using mouse-Chinese hamster somatic cell hybrids and haplotype analysis in interspecific backcross mice, mouse Pdgfra was mapped to chromosome 5 in tight linkage with Kit. Hybridization of a PDGFRA probe to DNAs from W(19H)/+ heterozygous mice and patch heterozygous mice, and their wild-type littermates, demonstrated deletion of Pdgfra. Pulsed-field gel electrophoresis indicated that Kit and Pdgfra are linked on a 630-kilobase Mlu I DNA fragment. Thus the W(19H) deletion removes at least two receptor tyrosine kinases and the results suggest Pdgfra as a candidate for the Ph locus.

Original language	English (US)
Pages (from-to)	4811-4815
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	88
Issue number	11
DOIs	https://doi.org/10.1073/pnas.88.11.4811
State	Published - Jan 1 1991

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Smith, E. A., Seldin, M. F., Martinez, L., Watson, M. L., Choudhury, G. G., Lalley, P. A., Pierce, J., Aaronson, S., Barker, J., Naylor, S. L., & Sakaguchi, A. Y. (1991). Mouse platelet-derived growth factor receptor α gene is deleted in W(19H) and patch mutations on chromosome 5. Proceedings of the National Academy of Sciences of the United States of America, 88(11), 4811-4815. https://doi.org/10.1073/pnas.88.11.4811

Mouse platelet-derived growth factor receptor α gene is deleted in W(19H) and patch mutations on chromosome 5. / Smith, E. A.; Seldin, M. F.; Martinez, L.; Watson, M. L.; Choudhury, G. G.; Lalley, P. A.; Pierce, J.; Aaronson, S.; Barker, J.; Naylor, S. L.; Sakaguchi, A. Y.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 11, 01.01.1991, p. 4811-4815.

Research output: Contribution to journal › Article

Smith, EA, Seldin, MF, Martinez, L, Watson, ML, Choudhury, GG, Lalley, PA, Pierce, J, Aaronson, S, Barker, J, Naylor, SL & Sakaguchi, AY 1991, 'Mouse platelet-derived growth factor receptor α gene is deleted in W(19H) and patch mutations on chromosome 5', Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 11, pp. 4811-4815. https://doi.org/10.1073/pnas.88.11.4811

Smith, E. A. ; Seldin, M. F. ; Martinez, L. ; Watson, M. L. ; Choudhury, G. G. ; Lalley, P. A. ; Pierce, J. ; Aaronson, S. ; Barker, J. ; Naylor, S. L. ; Sakaguchi, A. Y. / Mouse platelet-derived growth factor receptor α gene is deleted in W(19H) and patch mutations on chromosome 5. In: Proceedings of the National Academy of Sciences of the United States of America. 1991 ; Vol. 88, No. 11. pp. 4811-4815.

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abstract = "The mouse W(19H) mutation is an x-ray-induced deletion of more than 2 centimorgans on chromosome 5 encompassing the white spotting mutation W (encoded by the Kit protooncogene), patch (Ph), and recessive lethal (l) loci. The platelet-derived growth factor receptor α gene (PDGFRA) like Kit encodes a transmembrane receptor tyrosine kinase. By using mouse-Chinese hamster somatic cell hybrids and haplotype analysis in interspecific backcross mice, mouse Pdgfra was mapped to chromosome 5 in tight linkage with Kit. Hybridization of a PDGFRA probe to DNAs from W(19H)/+ heterozygous mice and patch heterozygous mice, and their wild-type littermates, demonstrated deletion of Pdgfra. Pulsed-field gel electrophoresis indicated that Kit and Pdgfra are linked on a 630-kilobase Mlu I DNA fragment. Thus the W(19H) deletion removes at least two receptor tyrosine kinases and the results suggest Pdgfra as a candidate for the Ph locus.",

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AU - Pierce, J.

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AU - Naylor, S. L.

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