Mouse model of subarachnoid hemorrhage associated cerebral vasospasm: Methodological analysis

A. Parra, M. J. McGirt, H. Sheng, D. T. Laskowitz, R. D. Pearlstein, D. S. Warner

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The transgenic mouse has been used to study subarachnoid hemorrhage (SAH) induced delayed cerebral vasospasm (DCV). Methodological parameters have not been analyzed to validate this model and associated neurological deficits have not been described. We introduce a technique to quantify DCV and associated neurological deficits. C57BL/6J mice were subjected to SAH or sham surgery. Seventy-two hours later, the vasculature was cast in situ with India ink/gelatin at perfusion pressures of 40-60, 60-80, or 100-120 mmHg. Mice were perfused with and without microfiltration. Additional mice underwent grading of SAH size, measurement of vascular diameters, and neurological examination (score range 5-27; 27=normal). When cast at 60-80 mmHg, SAH was associated with an intraluminal cross-sectional diameter reduction in 3 of 7 ipsilateral vascular segments. At 40-60 mmHg, the diameter of only one segment was reduced. No changes were observed at 100-120 mmHg. Emboli prevented adequate perfusion of vascular segments in the absence of microfiltration. Median (interquartile range) neurologic score was reduced after SAH (sham, 27 (27); SAH 11 (7-17)). Deficits correlated with middle cerebral artery (MCA) diameter and SAH grade. MCA diameter also correlated with SAH grade. Only when utilizing microfiltration, controlling for hemorrhage size, and casting at perfusion pressures of 60-80 mmHg does India ink/gelatin vascular casting demonstrate consistent DCV that correspnds to neurological deficits. This allows measurement of both anatomical and clinical DCV in the mouse.

Original languageEnglish (US)
Pages (from-to)510-516
Number of pages7
JournalNeurological Research
Volume24
Issue number5
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Intracranial Vasospasm
Subarachnoid Hemorrhage
Blood Vessels
Perfusion
Middle Cerebral Artery
Gelatin
Neurological Models
Pressure
Neurologic Examination
Embolism
Inbred C57BL Mouse
Nervous System
Transgenic Mice
Hemorrhage

Keywords

  • Brain
  • Mouse
  • Subarachnoid hemorrhage
  • Vasospasm

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Parra, A., McGirt, M. J., Sheng, H., Laskowitz, D. T., Pearlstein, R. D., & Warner, D. S. (2002). Mouse model of subarachnoid hemorrhage associated cerebral vasospasm: Methodological analysis. Neurological Research, 24(5), 510-516. https://doi.org/10.1179/016164102101200276

Mouse model of subarachnoid hemorrhage associated cerebral vasospasm : Methodological analysis. / Parra, A.; McGirt, M. J.; Sheng, H.; Laskowitz, D. T.; Pearlstein, R. D.; Warner, D. S.

In: Neurological Research, Vol. 24, No. 5, 2002, p. 510-516.

Research output: Contribution to journalArticle

Parra, A, McGirt, MJ, Sheng, H, Laskowitz, DT, Pearlstein, RD & Warner, DS 2002, 'Mouse model of subarachnoid hemorrhage associated cerebral vasospasm: Methodological analysis', Neurological Research, vol. 24, no. 5, pp. 510-516. https://doi.org/10.1179/016164102101200276
Parra, A. ; McGirt, M. J. ; Sheng, H. ; Laskowitz, D. T. ; Pearlstein, R. D. ; Warner, D. S. / Mouse model of subarachnoid hemorrhage associated cerebral vasospasm : Methodological analysis. In: Neurological Research. 2002 ; Vol. 24, No. 5. pp. 510-516.
@article{cd08da655bae48efaebeb503c250749a,
title = "Mouse model of subarachnoid hemorrhage associated cerebral vasospasm: Methodological analysis",
abstract = "The transgenic mouse has been used to study subarachnoid hemorrhage (SAH) induced delayed cerebral vasospasm (DCV). Methodological parameters have not been analyzed to validate this model and associated neurological deficits have not been described. We introduce a technique to quantify DCV and associated neurological deficits. C57BL/6J mice were subjected to SAH or sham surgery. Seventy-two hours later, the vasculature was cast in situ with India ink/gelatin at perfusion pressures of 40-60, 60-80, or 100-120 mmHg. Mice were perfused with and without microfiltration. Additional mice underwent grading of SAH size, measurement of vascular diameters, and neurological examination (score range 5-27; 27=normal). When cast at 60-80 mmHg, SAH was associated with an intraluminal cross-sectional diameter reduction in 3 of 7 ipsilateral vascular segments. At 40-60 mmHg, the diameter of only one segment was reduced. No changes were observed at 100-120 mmHg. Emboli prevented adequate perfusion of vascular segments in the absence of microfiltration. Median (interquartile range) neurologic score was reduced after SAH (sham, 27 (27); SAH 11 (7-17)). Deficits correlated with middle cerebral artery (MCA) diameter and SAH grade. MCA diameter also correlated with SAH grade. Only when utilizing microfiltration, controlling for hemorrhage size, and casting at perfusion pressures of 60-80 mmHg does India ink/gelatin vascular casting demonstrate consistent DCV that correspnds to neurological deficits. This allows measurement of both anatomical and clinical DCV in the mouse.",
keywords = "Brain, Mouse, Subarachnoid hemorrhage, Vasospasm",
author = "A. Parra and McGirt, {M. J.} and H. Sheng and Laskowitz, {D. T.} and Pearlstein, {R. D.} and Warner, {D. S.}",
year = "2002",
doi = "10.1179/016164102101200276",
language = "English (US)",
volume = "24",
pages = "510--516",
journal = "Neurological Research",
issn = "0161-6412",
publisher = "Maney Publishing",
number = "5",

}

TY - JOUR

T1 - Mouse model of subarachnoid hemorrhage associated cerebral vasospasm

T2 - Methodological analysis

AU - Parra, A.

AU - McGirt, M. J.

AU - Sheng, H.

AU - Laskowitz, D. T.

AU - Pearlstein, R. D.

AU - Warner, D. S.

PY - 2002

Y1 - 2002

N2 - The transgenic mouse has been used to study subarachnoid hemorrhage (SAH) induced delayed cerebral vasospasm (DCV). Methodological parameters have not been analyzed to validate this model and associated neurological deficits have not been described. We introduce a technique to quantify DCV and associated neurological deficits. C57BL/6J mice were subjected to SAH or sham surgery. Seventy-two hours later, the vasculature was cast in situ with India ink/gelatin at perfusion pressures of 40-60, 60-80, or 100-120 mmHg. Mice were perfused with and without microfiltration. Additional mice underwent grading of SAH size, measurement of vascular diameters, and neurological examination (score range 5-27; 27=normal). When cast at 60-80 mmHg, SAH was associated with an intraluminal cross-sectional diameter reduction in 3 of 7 ipsilateral vascular segments. At 40-60 mmHg, the diameter of only one segment was reduced. No changes were observed at 100-120 mmHg. Emboli prevented adequate perfusion of vascular segments in the absence of microfiltration. Median (interquartile range) neurologic score was reduced after SAH (sham, 27 (27); SAH 11 (7-17)). Deficits correlated with middle cerebral artery (MCA) diameter and SAH grade. MCA diameter also correlated with SAH grade. Only when utilizing microfiltration, controlling for hemorrhage size, and casting at perfusion pressures of 60-80 mmHg does India ink/gelatin vascular casting demonstrate consistent DCV that correspnds to neurological deficits. This allows measurement of both anatomical and clinical DCV in the mouse.

AB - The transgenic mouse has been used to study subarachnoid hemorrhage (SAH) induced delayed cerebral vasospasm (DCV). Methodological parameters have not been analyzed to validate this model and associated neurological deficits have not been described. We introduce a technique to quantify DCV and associated neurological deficits. C57BL/6J mice were subjected to SAH or sham surgery. Seventy-two hours later, the vasculature was cast in situ with India ink/gelatin at perfusion pressures of 40-60, 60-80, or 100-120 mmHg. Mice were perfused with and without microfiltration. Additional mice underwent grading of SAH size, measurement of vascular diameters, and neurological examination (score range 5-27; 27=normal). When cast at 60-80 mmHg, SAH was associated with an intraluminal cross-sectional diameter reduction in 3 of 7 ipsilateral vascular segments. At 40-60 mmHg, the diameter of only one segment was reduced. No changes were observed at 100-120 mmHg. Emboli prevented adequate perfusion of vascular segments in the absence of microfiltration. Median (interquartile range) neurologic score was reduced after SAH (sham, 27 (27); SAH 11 (7-17)). Deficits correlated with middle cerebral artery (MCA) diameter and SAH grade. MCA diameter also correlated with SAH grade. Only when utilizing microfiltration, controlling for hemorrhage size, and casting at perfusion pressures of 60-80 mmHg does India ink/gelatin vascular casting demonstrate consistent DCV that correspnds to neurological deficits. This allows measurement of both anatomical and clinical DCV in the mouse.

KW - Brain

KW - Mouse

KW - Subarachnoid hemorrhage

KW - Vasospasm

UR - http://www.scopus.com/inward/record.url?scp=0035985426&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035985426&partnerID=8YFLogxK

U2 - 10.1179/016164102101200276

DO - 10.1179/016164102101200276

M3 - Article

C2 - 12117325

AN - SCOPUS:0035985426

VL - 24

SP - 510

EP - 516

JO - Neurological Research

JF - Neurological Research

SN - 0161-6412

IS - 5

ER -