A cDNA clone for mouse immune interferon has been used to map the mouse interferon γ gene (Ifg)to a specific chromosome. This clone, which contains a 638-bp insert, detects an 18-kb HindIII fragment of mouse DNA. The presence of the mouse Ifg tgene in cell hybrids and its chromosomal location were determined by assaying cell hybrid DNA for the presence of the 18-kb HindIII fragment by Southern filter hybridization. Under the hybridization conditions used, Chinese hamster DNA did not hybridize to the cDNA probe. The segregation of mouse chromosomes in cell hybrids indicated that Ifg is located on chromosome 10. Previously, we have mapped immune interferon to the p12.05 → qter region of chromosome 12 in humans (1). This region of chromosome 12 also contains the genes for peptidase B and citrate synthase. The homologous genes in mouse are also located on chromosome 10, suggesting that these genes comprise a conserved linkage group.
ASJC Scopus subject areas
- Cell Biology