Abstract
Mouse skin contains a NADPH dependent, aryl hydrocarbon hydroxylase (AHH), which is inducible by polycyclic aromatic hydrocarbons. In general, unsubstituted polycyclic hydrocarbons caused a greater induction of epidermal AHH than substituted ones (1,2,3,4 dibenzanthracene > 1,2,5,6 dibenzanthracene > benz [a]anthracene ≥ 3 methylcholanthrene > > 7,12 dimethylbenz[a]anthracene) which did not correlate with their ability to initiate tumors in mouse skin. Two different techniques were used to isolate epidermis and similar results were obtained with both. However, the technique of isolating epidermis using a mild heat treatment required that the temperature be maintained at 52°C for 30 sec. If the temperature was raised to 54°C or above, there was a large reduction in the AHH activity. Isolated epidermis has 4 to 5 times the AHH activity as dermis and about twice that of whole skin. This was true for control mice in which AHH was induced by pretreatment with benz[a]anthracene.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 108-111 |
| Number of pages | 4 |
| Journal | Journal of Investigative Dermatology |
| Volume | 66 |
| Issue number | 2 |
| State | Published - 1976 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Dermatology
Cite this
Mouse epidermal aryl hydrocarbon hydroxylase. / Thompson, S.; Slaga, Thomas J.
In: Journal of Investigative Dermatology, Vol. 66, No. 2, 1976, p. 108-111.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mouse epidermal aryl hydrocarbon hydroxylase
AU - Thompson, S.
AU - Slaga, Thomas J
PY - 1976
Y1 - 1976
N2 - Mouse skin contains a NADPH dependent, aryl hydrocarbon hydroxylase (AHH), which is inducible by polycyclic aromatic hydrocarbons. In general, unsubstituted polycyclic hydrocarbons caused a greater induction of epidermal AHH than substituted ones (1,2,3,4 dibenzanthracene > 1,2,5,6 dibenzanthracene > benz [a]anthracene ≥ 3 methylcholanthrene > > 7,12 dimethylbenz[a]anthracene) which did not correlate with their ability to initiate tumors in mouse skin. Two different techniques were used to isolate epidermis and similar results were obtained with both. However, the technique of isolating epidermis using a mild heat treatment required that the temperature be maintained at 52°C for 30 sec. If the temperature was raised to 54°C or above, there was a large reduction in the AHH activity. Isolated epidermis has 4 to 5 times the AHH activity as dermis and about twice that of whole skin. This was true for control mice in which AHH was induced by pretreatment with benz[a]anthracene.
AB - Mouse skin contains a NADPH dependent, aryl hydrocarbon hydroxylase (AHH), which is inducible by polycyclic aromatic hydrocarbons. In general, unsubstituted polycyclic hydrocarbons caused a greater induction of epidermal AHH than substituted ones (1,2,3,4 dibenzanthracene > 1,2,5,6 dibenzanthracene > benz [a]anthracene ≥ 3 methylcholanthrene > > 7,12 dimethylbenz[a]anthracene) which did not correlate with their ability to initiate tumors in mouse skin. Two different techniques were used to isolate epidermis and similar results were obtained with both. However, the technique of isolating epidermis using a mild heat treatment required that the temperature be maintained at 52°C for 30 sec. If the temperature was raised to 54°C or above, there was a large reduction in the AHH activity. Isolated epidermis has 4 to 5 times the AHH activity as dermis and about twice that of whole skin. This was true for control mice in which AHH was induced by pretreatment with benz[a]anthracene.
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UR - http://www.scopus.com/inward/citedby.url?scp=0017284798&partnerID=8YFLogxK
M3 - Article
C2 - 1447
AN - SCOPUS:0017284798
VL - 66
SP - 108
EP - 111
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 2
ER -