Mosquito saliva alone has profound effects on the human immune system

Megan B. Vogt, Anismrita Lahon, Ravi P. Arya, Alexander R. Kneubehl, Jennifer L. Spencer Clinton, Silke Paust, Rebecca Rico-Hesse

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mosquito saliva is a very complex concoction of >100 proteins, many of which have unknown functions. The effects of mosquito saliva proteins injected into our skin during blood feeding have been studied mainly in mouse models of injection or biting, with many of these systems producing results that may not be relevant to human disease. Here, we describe the numerous effects that mosquito bites have on human immune cells in mice engrafted with human hematopoietic stem cells. We used flow cytometry and multiplex cytokine bead array assays, with detailed statistical analyses, to detect small but significant variations in immune cell functions after 4 mosquitoes fed on humanized mice footpads. After preliminary analyses, at different early times after biting, we focused on assessing innate immune and subsequent cellular responses at 6 hours, 24 hours and 7 days after mosquito bites. We detected both Th1 and Th2 human immune responses, and delayed effects on cytokine levels in the blood, and immune cell compositions in the skin and bone marrow, up to 7 days post-bites. These are the first measurements of this kind, with human immune responses in whole animals, bitten by living mosquitoes, versus previous studies using incomplete mouse models and salivary gland extracts or needle injected saliva. The results have major implications for the study of hematophagous insect saliva, its effects on the human immune system, with or without pathogen transmission, and the possibility of determining which of these proteins to target for vaccination, in attempts to block transmission of numerous tropical diseases.

Original languageEnglish (US)
Article numbere0006439
JournalPLoS Neglected Tropical Diseases
Volume12
Issue number5
DOIs
StatePublished - May 17 2018
Externally publishedYes

Fingerprint

Culicidae
Saliva
Immune System
Bites and Stings
Cytokines
Skin
Proteins
Infectious Disease Transmission
Hematopoietic Stem Cells
Salivary Glands
Cellular Immunity
Needles
Insects
Blood Cells
Flow Cytometry
Vaccination
Bone Marrow
Injections

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Vogt, M. B., Lahon, A., Arya, R. P., Kneubehl, A. R., Spencer Clinton, J. L., Paust, S., & Rico-Hesse, R. (2018). Mosquito saliva alone has profound effects on the human immune system. PLoS Neglected Tropical Diseases, 12(5), [e0006439]. https://doi.org/10.1371/journal.pntd.0006439

Mosquito saliva alone has profound effects on the human immune system. / Vogt, Megan B.; Lahon, Anismrita; Arya, Ravi P.; Kneubehl, Alexander R.; Spencer Clinton, Jennifer L.; Paust, Silke; Rico-Hesse, Rebecca.

In: PLoS Neglected Tropical Diseases, Vol. 12, No. 5, e0006439, 17.05.2018.

Research output: Contribution to journalArticle

Vogt, MB, Lahon, A, Arya, RP, Kneubehl, AR, Spencer Clinton, JL, Paust, S & Rico-Hesse, R 2018, 'Mosquito saliva alone has profound effects on the human immune system', PLoS Neglected Tropical Diseases, vol. 12, no. 5, e0006439. https://doi.org/10.1371/journal.pntd.0006439
Vogt MB, Lahon A, Arya RP, Kneubehl AR, Spencer Clinton JL, Paust S et al. Mosquito saliva alone has profound effects on the human immune system. PLoS Neglected Tropical Diseases. 2018 May 17;12(5). e0006439. https://doi.org/10.1371/journal.pntd.0006439
Vogt, Megan B. ; Lahon, Anismrita ; Arya, Ravi P. ; Kneubehl, Alexander R. ; Spencer Clinton, Jennifer L. ; Paust, Silke ; Rico-Hesse, Rebecca. / Mosquito saliva alone has profound effects on the human immune system. In: PLoS Neglected Tropical Diseases. 2018 ; Vol. 12, No. 5.
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