Mortality shifts in Caenorhabditis elegans: Remembrance of conditions past

Deqing Wu, Shane L. Rea, James R. Cypser, Thomas E. Johnson

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The analysis of age-specific mortality can yield insights into how anti-aging interventions operate that cannot be matched by simple assessment of longevity. Mortality, as opposed to longevity, can be used to assess the effects of an anti-aging intervention on a daily basis, rather than only after most animals have died. Various gerontogene mutations in Caenorhabditis elegans have been shown to increase longevity as much as tenfold and to decrease mortality at some ages even more. Environmental alterations, such as reduced food intake (dietary restriction) and lower temperature also result in reduced mortality soon after the intervention. Here, we ask how soon anti-aging interventions, applied during adult life, affect age-specific mortality in nematodes. Using maximum likelihood analysis, we estimated the Gompertz parameters after shifts of temperature, and of food concentration and maintenance conditions. In separate experiments, we altered expression of age-1 and daf-16, using RNAi. Using about 44 000 nematodes in total, to examine daily mortality, we find that for both types of environmental shift, mortality responded immediately in the first assessment, while RNAi-induced changes resulted in a slower response, perhaps due to delayed mechanics of RNAi action. However, under all conditions there is a permanent 'memory' of past states, such that the initial mortality component [a] of the Gompertz equation [μ(x) = aebx] bears a permanent 'imprint' of that earlier state. However, 'b' (the rate of mortality increase with age) is always specified by the current conditions.

Original languageEnglish (US)
Pages (from-to)666-675
Number of pages10
JournalAging cell
Volume8
Issue number6
DOIs
StatePublished - Dec 1 2009

Keywords

  • Aging
  • C. elegans
  • Caloric restriction
  • Demography
  • Genetics
  • Gerontogenes
  • Life tables
  • Lifespan
  • Longevity
  • Mortality
  • RNAi
  • Temperature shift

ASJC Scopus subject areas

  • Aging
  • Cell Biology

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