Morphology, physiological characteristics, and complete sequence of marine bacteriophage φ{phonetic}RIO-1 Infecting pseudoalteromonas marina

Stephen C. Hardies, Yeon J. Hwang, Chung Y. Hwang, Gwang I. Jang, Byung C. Cho

Research output: Contribution to journalArticle

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Abstract

Bacteria of the genus Pseudoalteromonas are ubiquitous in the world's oceans. Marine bacteria have been posited to be associ-ated with a major ancient branch of podoviruses related to T7. Yet, although Pseudoalteromonas phages belonging to the Corti-coviridae and the Siphoviridae and prophages belonging to the Myoviridae have been reported, no Pseudoalteromonas podovirus was previously known. Here, a new lytic Pseudoalteromonas marina phage, Φ{phonetic} RIO-1, belonging to the Podoviridae was isolated and characterized with respect to morphology, genomic sequence, and biological properties. Its major encoded proteins were distantly similar to those of T7. The most similar previously sequenced viruses were Pseudomonas phage PA11 and Salinivibrio phage CW02. Whereas many elements of the morphology and gene organization of Φ{phonetic} RIO-1 are similar to those of podoviruses broadly related to T7, Φ{phonetic} RIO-1 conspicuously lacked an RNA polymerase gene. Since definitions of a T7 supergroup have in-cluded similarity in the DNA polymerase gene, a detailed phylogenetic analysis was conducted, and two major DNA polymerase clades in Autographivirinae and several structural variants of the polA family represented in podoviruses were found. Φ{phonetic} RIO-1 carries an operon similar to that in a few other podoviruses predicted to specify activities related to MathematicalPi-Four.-1.H9253 -glutamyl amide linkages and/or unusual peptide bonds. Most growth properties of Φ{phonetic} RIO-1 were typical of T7-like phages, except for a long latent period.

Original languageEnglish (US)
Pages (from-to)9189-9198
Number of pages10
JournalJournal of virology
Volume87
Issue number16
DOIs
StatePublished - Aug 1 2013

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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