Morphological changes of the nucleolar organizer regions induced by 7,12-dimethylbenz[a]anthracene in the hamster cheek pouch

N. Yoshimi, I. B. Gimenez-Conti, Thomas J Slaga

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The staining of nucleolar organizer regions (NORs), which equate to rDNA transcription, was applied to chemically induced-lesions of the hamster cheek pouch. The cheek pouches of 16 male golden Syrian hamsters were treated three times a week with 0.5% 7,12-dimethylbenz[a]anthracene (DMBA) in mineral oil for 16 weeks. The percentage of gathered-type NORs with high activity nucleoli increased in the pouch epithelium during DMBA treatment and reached the highest values in malignant tumors. The percentage of dispersed-type NORs also increased in the malignant lesions. However, the absolute number of NORs was not affected by DMBA treatment. These results suggest that DMBA induces modification of NOR activity at the early stages of carcinogenesis and shows the potential of this model for studying NOR alterations in neoplasia.

Original languageEnglish (US)
Pages (from-to)97-100
Number of pages4
JournalJournal of Oral Pathology and Medicine
Volume22
Issue number3
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Nucleolus Organizer Region
Cheek
Cricetinae
9,10-Dimethyl-1,2-benzanthracene
Mineral Oil
Mesocricetus
Ribosomal DNA
anthracene
Neoplasms
Carcinogenesis
Epithelium
Staining and Labeling

Keywords

  • carcinogenesis by DMBA
  • hamster cheek pouch
  • nucleolar organizer regions

ASJC Scopus subject areas

  • Cancer Research
  • Pathology and Forensic Medicine
  • Dentistry(all)

Cite this

Morphological changes of the nucleolar organizer regions induced by 7,12-dimethylbenz[a]anthracene in the hamster cheek pouch. / Yoshimi, N.; Gimenez-Conti, I. B.; Slaga, Thomas J.

In: Journal of Oral Pathology and Medicine, Vol. 22, No. 3, 1993, p. 97-100.

Research output: Contribution to journalArticle

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