Morphologic and microbiologic features of Pseudomonas aeruginosa pneumonia in normal hamsters

Jacqueline J. Coalson, Junji H. Higuchi, Mary L. Williams, Waldemar G. Johanson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A model of pneumonia due to Pseudomonas aeruginosa was produced in hamsters by an intratracheal bolus instillation of microorganisms. Sequential lung changes from 4 hr through 11 days were studied by morphologic and microbiologic methods. Hamsters inoculated with > 106 pseudomonads survived but consistently had histologic evidence of mild bronchopneumonia 24 hr postinoculation, whereas a severe bronchopneumonia and a 100% mortality were elicited with a 108 inoculum of organisms in 0.5 ml phosphate-buffered saline (PBS). An inoculum of 107 pseudomonads/0.5 ml PBS was then used to define the changes in the bacterial population in Pseudomonas pneumonia and to obtain serial histopathologic observations. Quantitative lung cultures obtained within 1 hr postinoculation demonstrated a mean of 106 colony forming units per lung, and none of the hamsters were bacteremic. However, by 24 hr bacterial counts had increased and all animals were bacteremic. Bacterial proliferation continued through 48 hr; however, the number of bacteremic animals had decreased. By 72 hr, bacterial counts had decreased with total Pseudmonas clearance noted by 120 hr. A striking polymorphonuclear leukocyte-rich alveolar exudate was present by 12 hr. Pseudomonas "vasculitis" was evident by 24 hr. The evolution of this vascular lesion correlated with the bacteremic state of the hamsters. By 11 days, resolution of the pneumonic process was seen. The macroscopic and microscopic features of this hamster model of Pseudomonas pneumonia are very similar to those reported in infected patients.

Original languageEnglish (US)
Pages (from-to)193-206
Number of pages14
JournalExperimental and Molecular Pathology
Volume45
Issue number2
DOIs
StatePublished - 1986

Fingerprint

Cricetinae
Pseudomonas aeruginosa
Pneumonia
Animals
Phosphates
Pseudomonas
Bronchopneumonia
Lung
Microorganisms
Bacterial Load
Exudates and Transudates
Vasculitis
Blood Vessels
Neutrophils
Stem Cells
Mortality
Population

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine

Cite this

Morphologic and microbiologic features of Pseudomonas aeruginosa pneumonia in normal hamsters. / Coalson, Jacqueline J.; Higuchi, Junji H.; Williams, Mary L.; Johanson, Waldemar G.

In: Experimental and Molecular Pathology, Vol. 45, No. 2, 1986, p. 193-206.

Research output: Contribution to journalArticle

Coalson, Jacqueline J. ; Higuchi, Junji H. ; Williams, Mary L. ; Johanson, Waldemar G. / Morphologic and microbiologic features of Pseudomonas aeruginosa pneumonia in normal hamsters. In: Experimental and Molecular Pathology. 1986 ; Vol. 45, No. 2. pp. 193-206.
@article{88b918955faf4c769b3a3e64818e136d,
title = "Morphologic and microbiologic features of Pseudomonas aeruginosa pneumonia in normal hamsters",
abstract = "A model of pneumonia due to Pseudomonas aeruginosa was produced in hamsters by an intratracheal bolus instillation of microorganisms. Sequential lung changes from 4 hr through 11 days were studied by morphologic and microbiologic methods. Hamsters inoculated with > 106 pseudomonads survived but consistently had histologic evidence of mild bronchopneumonia 24 hr postinoculation, whereas a severe bronchopneumonia and a 100{\%} mortality were elicited with a 108 inoculum of organisms in 0.5 ml phosphate-buffered saline (PBS). An inoculum of 107 pseudomonads/0.5 ml PBS was then used to define the changes in the bacterial population in Pseudomonas pneumonia and to obtain serial histopathologic observations. Quantitative lung cultures obtained within 1 hr postinoculation demonstrated a mean of 106 colony forming units per lung, and none of the hamsters were bacteremic. However, by 24 hr bacterial counts had increased and all animals were bacteremic. Bacterial proliferation continued through 48 hr; however, the number of bacteremic animals had decreased. By 72 hr, bacterial counts had decreased with total Pseudmonas clearance noted by 120 hr. A striking polymorphonuclear leukocyte-rich alveolar exudate was present by 12 hr. Pseudomonas {"}vasculitis{"} was evident by 24 hr. The evolution of this vascular lesion correlated with the bacteremic state of the hamsters. By 11 days, resolution of the pneumonic process was seen. The macroscopic and microscopic features of this hamster model of Pseudomonas pneumonia are very similar to those reported in infected patients.",
author = "Coalson, {Jacqueline J.} and Higuchi, {Junji H.} and Williams, {Mary L.} and Johanson, {Waldemar G.}",
year = "1986",
doi = "10.1016/0014-4800(86)90059-6",
language = "English (US)",
volume = "45",
pages = "193--206",
journal = "Experimental and Molecular Pathology",
issn = "0014-4800",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Morphologic and microbiologic features of Pseudomonas aeruginosa pneumonia in normal hamsters

AU - Coalson, Jacqueline J.

AU - Higuchi, Junji H.

AU - Williams, Mary L.

AU - Johanson, Waldemar G.

PY - 1986

Y1 - 1986

N2 - A model of pneumonia due to Pseudomonas aeruginosa was produced in hamsters by an intratracheal bolus instillation of microorganisms. Sequential lung changes from 4 hr through 11 days were studied by morphologic and microbiologic methods. Hamsters inoculated with > 106 pseudomonads survived but consistently had histologic evidence of mild bronchopneumonia 24 hr postinoculation, whereas a severe bronchopneumonia and a 100% mortality were elicited with a 108 inoculum of organisms in 0.5 ml phosphate-buffered saline (PBS). An inoculum of 107 pseudomonads/0.5 ml PBS was then used to define the changes in the bacterial population in Pseudomonas pneumonia and to obtain serial histopathologic observations. Quantitative lung cultures obtained within 1 hr postinoculation demonstrated a mean of 106 colony forming units per lung, and none of the hamsters were bacteremic. However, by 24 hr bacterial counts had increased and all animals were bacteremic. Bacterial proliferation continued through 48 hr; however, the number of bacteremic animals had decreased. By 72 hr, bacterial counts had decreased with total Pseudmonas clearance noted by 120 hr. A striking polymorphonuclear leukocyte-rich alveolar exudate was present by 12 hr. Pseudomonas "vasculitis" was evident by 24 hr. The evolution of this vascular lesion correlated with the bacteremic state of the hamsters. By 11 days, resolution of the pneumonic process was seen. The macroscopic and microscopic features of this hamster model of Pseudomonas pneumonia are very similar to those reported in infected patients.

AB - A model of pneumonia due to Pseudomonas aeruginosa was produced in hamsters by an intratracheal bolus instillation of microorganisms. Sequential lung changes from 4 hr through 11 days were studied by morphologic and microbiologic methods. Hamsters inoculated with > 106 pseudomonads survived but consistently had histologic evidence of mild bronchopneumonia 24 hr postinoculation, whereas a severe bronchopneumonia and a 100% mortality were elicited with a 108 inoculum of organisms in 0.5 ml phosphate-buffered saline (PBS). An inoculum of 107 pseudomonads/0.5 ml PBS was then used to define the changes in the bacterial population in Pseudomonas pneumonia and to obtain serial histopathologic observations. Quantitative lung cultures obtained within 1 hr postinoculation demonstrated a mean of 106 colony forming units per lung, and none of the hamsters were bacteremic. However, by 24 hr bacterial counts had increased and all animals were bacteremic. Bacterial proliferation continued through 48 hr; however, the number of bacteremic animals had decreased. By 72 hr, bacterial counts had decreased with total Pseudmonas clearance noted by 120 hr. A striking polymorphonuclear leukocyte-rich alveolar exudate was present by 12 hr. Pseudomonas "vasculitis" was evident by 24 hr. The evolution of this vascular lesion correlated with the bacteremic state of the hamsters. By 11 days, resolution of the pneumonic process was seen. The macroscopic and microscopic features of this hamster model of Pseudomonas pneumonia are very similar to those reported in infected patients.

UR - http://www.scopus.com/inward/record.url?scp=0023009770&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023009770&partnerID=8YFLogxK

U2 - 10.1016/0014-4800(86)90059-6

DO - 10.1016/0014-4800(86)90059-6

M3 - Article

C2 - 3770145

AN - SCOPUS:0023009770

VL - 45

SP - 193

EP - 206

JO - Experimental and Molecular Pathology

JF - Experimental and Molecular Pathology

SN - 0014-4800

IS - 2

ER -