Morbid obesity resulting from inactivation of the ciliary protein CEP19 in humans and mice

Adel Shalata, Maria C. Ramirez, Robert J. Desnick, Nolan Priedigkeit, Christoph Buettner, Claudia Lindtner, Mohammed Mahroum, Muhammad A Abdul-ghani, Feng Dong, Nazik Arar, Olga Camacho-Vanegas, Rui Zhang, Sandra C. Camacho, Ying Chen, Mwafaq Ibdah, Ralph A Defronzo, Virginia Gillespie, Kevin Kelley, Brian D. Dynlacht, Sehyun Kim & 3 others Marc J. Glucksman, Zvi U. Borochowitz, John A. Martignetti

Research output: Contribution to journalArticle

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Abstract

Obesity is a major public health concern, and complementary research strategies have been directed toward the identification of the underlying causative gene mutations that affect the normal pathways and networks that regulate energy balance. Here, we describe an autosomal-recessive morbid-obesity syndrome and identify the disease-causing gene defect. The average body mass index of affected family members was 48.7 (range = 36.7-61.0), and all had features of the metabolic syndrome. Homozygosity mapping localized the disease locus to a region in 3q29; we designated this region the morbid obesity 1 (MO1) locus. Sequence analysis identified a homozygous nonsense mutation in CEP19, the gene encoding the ciliary protein CEP19, in all affected family members. CEP19 is highly conserved in vertebrates and invertebrates, is expressed in multiple tissues, and localizes to the centrosome and primary cilia. Homozygous Cep19-knockout mice were morbidly obese, hyperphagic, glucose intolerant, and insulin resistant. Thus, loss of the ciliary protein CEP19 in humans and mice causes morbid obesity and defines a target for investigating the molecular pathogenesis of this disease and potential treatments for obesity and malnutrition.

Original languageEnglish (US)
Pages (from-to)1061-1071
Number of pages11
JournalAmerican Journal of Human Genetics
Volume93
Issue number6
DOIs
StatePublished - Dec 5 2013

Fingerprint

Morbid Obesity
Obesity
Genes
Centrosome
Cilia
Nonsense Codon
Invertebrates
Knockout Mice
Malnutrition
Sequence Analysis
Vertebrates
Body Mass Index
Public Health
Insulin
Glucose
Mutation
Research
mouse CEP19 protein
human CEP19 protein
Proteins

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Shalata, A., Ramirez, M. C., Desnick, R. J., Priedigkeit, N., Buettner, C., Lindtner, C., ... Martignetti, J. A. (2013). Morbid obesity resulting from inactivation of the ciliary protein CEP19 in humans and mice. American Journal of Human Genetics, 93(6), 1061-1071. https://doi.org/10.1016/j.ajhg.2013.10.025

Morbid obesity resulting from inactivation of the ciliary protein CEP19 in humans and mice. / Shalata, Adel; Ramirez, Maria C.; Desnick, Robert J.; Priedigkeit, Nolan; Buettner, Christoph; Lindtner, Claudia; Mahroum, Mohammed; Abdul-ghani, Muhammad A; Dong, Feng; Arar, Nazik; Camacho-Vanegas, Olga; Zhang, Rui; Camacho, Sandra C.; Chen, Ying; Ibdah, Mwafaq; Defronzo, Ralph A; Gillespie, Virginia; Kelley, Kevin; Dynlacht, Brian D.; Kim, Sehyun; Glucksman, Marc J.; Borochowitz, Zvi U.; Martignetti, John A.

In: American Journal of Human Genetics, Vol. 93, No. 6, 05.12.2013, p. 1061-1071.

Research output: Contribution to journalArticle

Shalata, A, Ramirez, MC, Desnick, RJ, Priedigkeit, N, Buettner, C, Lindtner, C, Mahroum, M, Abdul-ghani, MA, Dong, F, Arar, N, Camacho-Vanegas, O, Zhang, R, Camacho, SC, Chen, Y, Ibdah, M, Defronzo, RA, Gillespie, V, Kelley, K, Dynlacht, BD, Kim, S, Glucksman, MJ, Borochowitz, ZU & Martignetti, JA 2013, 'Morbid obesity resulting from inactivation of the ciliary protein CEP19 in humans and mice', American Journal of Human Genetics, vol. 93, no. 6, pp. 1061-1071. https://doi.org/10.1016/j.ajhg.2013.10.025
Shalata A, Ramirez MC, Desnick RJ, Priedigkeit N, Buettner C, Lindtner C et al. Morbid obesity resulting from inactivation of the ciliary protein CEP19 in humans and mice. American Journal of Human Genetics. 2013 Dec 5;93(6):1061-1071. https://doi.org/10.1016/j.ajhg.2013.10.025
Shalata, Adel ; Ramirez, Maria C. ; Desnick, Robert J. ; Priedigkeit, Nolan ; Buettner, Christoph ; Lindtner, Claudia ; Mahroum, Mohammed ; Abdul-ghani, Muhammad A ; Dong, Feng ; Arar, Nazik ; Camacho-Vanegas, Olga ; Zhang, Rui ; Camacho, Sandra C. ; Chen, Ying ; Ibdah, Mwafaq ; Defronzo, Ralph A ; Gillespie, Virginia ; Kelley, Kevin ; Dynlacht, Brian D. ; Kim, Sehyun ; Glucksman, Marc J. ; Borochowitz, Zvi U. ; Martignetti, John A. / Morbid obesity resulting from inactivation of the ciliary protein CEP19 in humans and mice. In: American Journal of Human Genetics. 2013 ; Vol. 93, No. 6. pp. 1061-1071.
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