TY - JOUR
T1 - Monocytic differentiation induction of HL-60 cells by MC 903, a novel vitamin D analogue
AU - Rebel, Vivienne I.
AU - Ossenkoppele, Gert J.
AU - van de Loosdrecht, Arjan A.
AU - Wijermans, Pierre W.
AU - Beelen, Robert H.J.
AU - Langenhuijsen, Mart M.A.C.
PY - 1992/5
Y1 - 1992/5
N2 - 1.25 (OH)2D3 is a potent inducer of differentiation of leukaemic cells into a monocytic direction. However, therapeutic application is difficult because of the development of hypercalcaemia. We examined a novel vitamin D analogue, MC 903, which is at least 100 times less effective on calcium metabolism in rats than 1.25 (OH)2D3. Using the HL-60 cell line, differentiation was measured with a comprehensive panel of qualitative and quantitative parameters. Development of monocytic cells was shown morphologically, immunophenotypically and functionally by increased capability of reducing NBT (vs cultures without MC 903, p < 0.0001) and by qualitatively and quantitatively increased non-specific esterase activity. Furthermore, a concomitant decreased activity of myeloperoxidase and lactate dehydrogenase was noticed. In conclusion, MC 903 is a potent inducer of monocytic differentiation, comparable with 1.25 (OH)2D3 and will therefore be an interesting and potential therapeutic agent for studies in human acute leukaemia.
AB - 1.25 (OH)2D3 is a potent inducer of differentiation of leukaemic cells into a monocytic direction. However, therapeutic application is difficult because of the development of hypercalcaemia. We examined a novel vitamin D analogue, MC 903, which is at least 100 times less effective on calcium metabolism in rats than 1.25 (OH)2D3. Using the HL-60 cell line, differentiation was measured with a comprehensive panel of qualitative and quantitative parameters. Development of monocytic cells was shown morphologically, immunophenotypically and functionally by increased capability of reducing NBT (vs cultures without MC 903, p < 0.0001) and by qualitatively and quantitatively increased non-specific esterase activity. Furthermore, a concomitant decreased activity of myeloperoxidase and lactate dehydrogenase was noticed. In conclusion, MC 903 is a potent inducer of monocytic differentiation, comparable with 1.25 (OH)2D3 and will therefore be an interesting and potential therapeutic agent for studies in human acute leukaemia.
KW - HL-60 cells
KW - MC 903
KW - monocytic differentiation
UR - http://www.scopus.com/inward/record.url?scp=0026767083&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026767083&partnerID=8YFLogxK
U2 - 10.1016/0145-2126(92)90169-8
DO - 10.1016/0145-2126(92)90169-8
M3 - Article
C2 - 1625469
AN - SCOPUS:0026767083
VL - 16
SP - 443
EP - 451
JO - Leukemia Research
JF - Leukemia Research
SN - 0145-2126
IS - 5
ER -