Monocyte rescue of human T cells from apoptosis is CD40/CD154 dependent

K. M. Sakata, A. Sakata, L. Kong, N. Vela-Roch, N. Talal, Howard Dang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The induction of T-cell apoptosis is regulated in part by monocytes (CD14+ cells). Human peripheral blood monocytes inhibited the spontaneous cell death of activated T cells in vitro. The inhibition of T-cell apoptosis did not require autologous monocytes. Inhibition required direct contact with monocytes and was not due to a soluble factor. Furthermore, treatment of monocytes with actinomycin D, cycloheximide and paraformaldehyde abrogated the anti-apoptotic activity of these cells. Blocking antibody to CD40 and CD154 (CD40 ligand) decreased the ability of monocytes to aid in T-cell survival, whereas, blocking LFA-1/I-CAM-1, Fas ligand and the CD4/major histocompatibility complex class II interaction did not affect the influence of monocytes on T-cell survival. This shows that monocytes rescue of activated T cells from apoptosis is dependent upon CD40/CD154 interaction.

Original languageEnglish (US)
Pages (from-to)479-484
Number of pages6
JournalScandinavian Journal of Immunology
Volume50
Issue number5
DOIs
StatePublished - 1999

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Monocytes
Apoptosis
T-Lymphocytes
Cell Survival
Lymphocyte Function-Associated Antigen-1
CD40 Ligand
Blocking Antibodies
Fas Ligand Protein
Dactinomycin
Cycloheximide
Major Histocompatibility Complex
Cell Death

ASJC Scopus subject areas

  • Immunology

Cite this

Sakata, K. M., Sakata, A., Kong, L., Vela-Roch, N., Talal, N., & Dang, H. (1999). Monocyte rescue of human T cells from apoptosis is CD40/CD154 dependent. Scandinavian Journal of Immunology, 50(5), 479-484. https://doi.org/10.1046/j.1365-3083.1999.00629.x

Monocyte rescue of human T cells from apoptosis is CD40/CD154 dependent. / Sakata, K. M.; Sakata, A.; Kong, L.; Vela-Roch, N.; Talal, N.; Dang, Howard.

In: Scandinavian Journal of Immunology, Vol. 50, No. 5, 1999, p. 479-484.

Research output: Contribution to journalArticle

Sakata, KM, Sakata, A, Kong, L, Vela-Roch, N, Talal, N & Dang, H 1999, 'Monocyte rescue of human T cells from apoptosis is CD40/CD154 dependent', Scandinavian Journal of Immunology, vol. 50, no. 5, pp. 479-484. https://doi.org/10.1046/j.1365-3083.1999.00629.x
Sakata, K. M. ; Sakata, A. ; Kong, L. ; Vela-Roch, N. ; Talal, N. ; Dang, Howard. / Monocyte rescue of human T cells from apoptosis is CD40/CD154 dependent. In: Scandinavian Journal of Immunology. 1999 ; Vol. 50, No. 5. pp. 479-484.
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