Monocyte rescue of human T cells from apoptosis is CD40/CD154 dependent

K. M. Sakata, A. Sakata, L. Kong, N. Vela-Roch, N. Talal, Howard Dang

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The induction of T-cell apoptosis is regulated in part by monocytes (CD14+ cells). Human peripheral blood monocytes inhibited the spontaneous cell death of activated T cells in vitro. The inhibition of T-cell apoptosis did not require autologous monocytes. Inhibition required direct contact with monocytes and was not due to a soluble factor. Furthermore, treatment of monocytes with actinomycin D, cycloheximide and paraformaldehyde abrogated the anti-apoptotic activity of these cells. Blocking antibody to CD40 and CD154 (CD40 ligand) decreased the ability of monocytes to aid in T-cell survival, whereas, blocking LFA-1/I-CAM-1, Fas ligand and the CD4/major histocompatibility complex class II interaction did not affect the influence of monocytes on T-cell survival. This shows that monocytes rescue of activated T cells from apoptosis is dependent upon CD40/CD154 interaction.

Original languageEnglish (US)
Pages (from-to)479-484
Number of pages6
JournalScandinavian Journal of Immunology
Volume50
Issue number5
DOIs
StatePublished - 1999

ASJC Scopus subject areas

  • Immunology

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    Sakata, K. M., Sakata, A., Kong, L., Vela-Roch, N., Talal, N., & Dang, H. (1999). Monocyte rescue of human T cells from apoptosis is CD40/CD154 dependent. Scandinavian Journal of Immunology, 50(5), 479-484. https://doi.org/10.1046/j.1365-3083.1999.00629.x