Monoclonal antibodies to α2(u)-globulin and immunocytofluorometric analysis of α2(u)-globulin-synthesizing hepatocytes during androgenic induction and aging

N. M. Motwani, D. Caron, W. F. Demyan, B. Chatterjee, S. Hunter, M. D. Poulik, A. K. Roy

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Stable hybridomas generated by fusion of spleen cells from hyperimmunized mice and mouse myeloma cells were cloned to prepare monoclonal antibodies to α2(u)-globulin, an androgen-dependent urinary protein of hepatic origin. One of these monoclonal antibodies was used as a probe for immunocytofluorometric analysis of α2(u)-globulin producing hepatocytes during androgenic induction and aging through fluorescence-activated cell sorting (FACS). FACS patterns of hepatocytes from mature male rats that produce high levels of α2(u)-globulin showed two distinct peaks, arbitrarily designated as peak I (weakly fluorescent) and peak II (brightly fluorescent). In the mature male rat, peak II represented about 40% of the total hepatocytes, and the fluorescence intensity of this subpopulation decreased in direct correspondence with the gradual decline of α2(u)-globulin synthesis during aging. Similarly the andrenogenic induction of this protein in ovariectomized female rats was associated with an increase in the fluorescence intensity of the hepatocyte subpopulation under peak II rather than an increase in the relative number of these cells. From these results we conclude that the androgen-dependent synthesis of α2(u)-globulin and its alteration during aging are confined to a specific subpopulation of hepatocytes within the liver.

Original languageEnglish (US)
Pages (from-to)3653-3657
Number of pages5
JournalJournal of Biological Chemistry
Volume259
Issue number6
StatePublished - May 24 1984
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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