Monoclonal antibodies define antigenic variation in the ID variety of Venezuelan equine encephalitis virus

R. Rico-Hesse, J. T. Roehrig, R. W. Dickerman

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    Abstract

    Three monoclonal antibodies were generated that are specific for the E2 glycoprotein of Venezuelan equine encephalitis (VEE) virus and have useful reactivities in an enzyme-linked immunosorbent assay (ELISA). Antibody 1A1B-9 distinguished between the IC (epizootic) and ID (enzootic) varieties of VEE virus by ELISA. Clone 7A1A-1 antibody distinguished the Panamanian prototype virus (3880) from Colombian ID isolates by a 500-fold difference in titer by endpoint ELISA, and it detected antigenic variation in ID isolates from southern California and Ecuador. Antibody 7A3A-4 defined a cryptic antigenic site on the latter two isolates. These monoclonal antibodies complement others in identifying VEE isolates by a simple ELISA.

    Original languageEnglish (US)
    Pages (from-to)187-194
    Number of pages8
    JournalAmerican Journal of Tropical Medicine and Hygiene
    Volume38
    Issue number1
    DOIs
    StatePublished - Jan 1 1988

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    ASJC Scopus subject areas

    • Parasitology
    • Virology
    • Infectious Diseases

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