Abstract
Three monoclonal antibodies were generated that are specific for the E2 glycoprotein of Venezuelan equine encephalitis (VEE) virus and have useful reactivities in an enzyme-linked immunosorbent assay (ELISA). Antibody 1A1B-9 distinguished between the IC (epizootic) and ID (enzootic) varieties of VEE virus by ELISA. Clone 7A1A-1 antibody distinguished the Panamanian prototype virus (3880) from Colombian ID isolates by a 500-fold difference in titer by endpoint ELISA, and it detected antigenic variation in ID isolates from southern California and Ecuador. Antibody 7A3A-4 defined a cryptic antigenic site on the latter two isolates. These monoclonal antibodies complement others in identifying VEE isolates by a simple ELISA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 187-194 |
| Number of pages | 8 |
| Journal | American Journal of Tropical Medicine and Hygiene |
| Volume | 38 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1988 |
ASJC Scopus subject areas
- Parasitology
- Virology
- Infectious Diseases