Monoclonal antibodies define antigenic variation in the ID variety of Venezuelan equine encephalitis virus

R. Rico-Hesse, J. T. Roehrig, R. W. Dickerman

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Three monoclonal antibodies were generated that are specific for the E2 glycoprotein of Venezuelan equine encephalitis (VEE) virus and have useful reactivities in an enzyme-linked immunosorbent assay (ELISA). Antibody 1A1B-9 distinguished between the IC (epizootic) and ID (enzootic) varieties of VEE virus by ELISA. Clone 7A1A-1 antibody distinguished the Panamanian prototype virus (3880) from Colombian ID isolates by a 500-fold difference in titer by endpoint ELISA, and it detected antigenic variation in ID isolates from southern California and Ecuador. Antibody 7A3A-4 defined a cryptic antigenic site on the latter two isolates. These monoclonal antibodies complement others in identifying VEE isolates by a simple ELISA.

Original languageEnglish (US)
Pages (from-to)187-194
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume38
Issue number1
StatePublished - 1988
Externally publishedYes

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Venezuelan Equine Encephalitis Viruses
Antigenic Variation
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Antibodies
Venezuelan Equine Encephalomyelitides
Ecuador
Glycoproteins
Clone Cells
Viruses

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Monoclonal antibodies define antigenic variation in the ID variety of Venezuelan equine encephalitis virus. / Rico-Hesse, R.; Roehrig, J. T.; Dickerman, R. W.

In: American Journal of Tropical Medicine and Hygiene, Vol. 38, No. 1, 1988, p. 187-194.

Research output: Contribution to journalArticle

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