Monoclonal antibodies define antigenic variation in the ID variety of Venezuelan equine encephalitis virus

R. Rico-Hesse; J. T. Roehrig; R. W. Dickerman

doi:10.4269/ajtmh.1988.38.187

Monoclonal antibodies define antigenic variation in the ID variety of Venezuelan equine encephalitis virus

R. Rico-Hesse, J. T. Roehrig, R. W. Dickerman

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17 Scopus citations

Abstract

Three monoclonal antibodies were generated that are specific for the E2 glycoprotein of Venezuelan equine encephalitis (VEE) virus and have useful reactivities in an enzyme-linked immunosorbent assay (ELISA). Antibody 1A1B-9 distinguished between the IC (epizootic) and ID (enzootic) varieties of VEE virus by ELISA. Clone 7A1A-1 antibody distinguished the Panamanian prototype virus (3880) from Colombian ID isolates by a 500-fold difference in titer by endpoint ELISA, and it detected antigenic variation in ID isolates from southern California and Ecuador. Antibody 7A3A-4 defined a cryptic antigenic site on the latter two isolates. These monoclonal antibodies complement others in identifying VEE isolates by a simple ELISA.

Original language	English (US)
Pages (from-to)	187-194
Number of pages	8
Journal	American Journal of Tropical Medicine and Hygiene
Volume	38
Issue number	1
DOIs	https://doi.org/10.4269/ajtmh.1988.38.187
State	Published - Jan 1 1988

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ASJC Scopus subject areas

Parasitology
Virology
Infectious Diseases

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Rico-Hesse, R., Roehrig, J. T., & Dickerman, R. W. (1988). Monoclonal antibodies define antigenic variation in the ID variety of Venezuelan equine encephalitis virus. American Journal of Tropical Medicine and Hygiene, 38(1), 187-194. https://doi.org/10.4269/ajtmh.1988.38.187

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10.4269/ajtmh.1988.38.187