Abstract
Three monoclonal antibodies were generated that are specific for the E2 glycoprotein of Venezuelan equine encephalitis (VEE) virus and have useful reactivities in an enzyme-linked immunosorbent assay (ELISA). Antibody 1A1B-9 distinguished between the IC (epizootic) and ID (enzootic) varieties of VEE virus by ELISA. Clone 7A1A-1 antibody distinguished the Panamanian prototype virus (3880) from Colombian ID isolates by a 500-fold difference in titer by endpoint ELISA, and it detected antigenic variation in ID isolates from southern California and Ecuador. Antibody 7A3A-4 defined a cryptic antigenic site on the latter two isolates. These monoclonal antibodies complement others in identifying VEE isolates by a simple ELISA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 187-194 |
| Number of pages | 8 |
| Journal | American Journal of Tropical Medicine and Hygiene |
| Volume | 38 |
| Issue number | 1 |
| State | Published - 1988 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Parasitology
- Infectious Diseases
Cite this
Monoclonal antibodies define antigenic variation in the ID variety of Venezuelan equine encephalitis virus. / Rico-Hesse, R.; Roehrig, J. T.; Dickerman, R. W.
In: American Journal of Tropical Medicine and Hygiene, Vol. 38, No. 1, 1988, p. 187-194.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Monoclonal antibodies define antigenic variation in the ID variety of Venezuelan equine encephalitis virus
AU - Rico-Hesse, R.
AU - Roehrig, J. T.
AU - Dickerman, R. W.
PY - 1988
Y1 - 1988
N2 - Three monoclonal antibodies were generated that are specific for the E2 glycoprotein of Venezuelan equine encephalitis (VEE) virus and have useful reactivities in an enzyme-linked immunosorbent assay (ELISA). Antibody 1A1B-9 distinguished between the IC (epizootic) and ID (enzootic) varieties of VEE virus by ELISA. Clone 7A1A-1 antibody distinguished the Panamanian prototype virus (3880) from Colombian ID isolates by a 500-fold difference in titer by endpoint ELISA, and it detected antigenic variation in ID isolates from southern California and Ecuador. Antibody 7A3A-4 defined a cryptic antigenic site on the latter two isolates. These monoclonal antibodies complement others in identifying VEE isolates by a simple ELISA.
AB - Three monoclonal antibodies were generated that are specific for the E2 glycoprotein of Venezuelan equine encephalitis (VEE) virus and have useful reactivities in an enzyme-linked immunosorbent assay (ELISA). Antibody 1A1B-9 distinguished between the IC (epizootic) and ID (enzootic) varieties of VEE virus by ELISA. Clone 7A1A-1 antibody distinguished the Panamanian prototype virus (3880) from Colombian ID isolates by a 500-fold difference in titer by endpoint ELISA, and it detected antigenic variation in ID isolates from southern California and Ecuador. Antibody 7A3A-4 defined a cryptic antigenic site on the latter two isolates. These monoclonal antibodies complement others in identifying VEE isolates by a simple ELISA.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023854477&partnerID=8YFLogxK
M3 - Article
C2 - 2449089
AN - SCOPUS:0023854477
VL - 38
SP - 187
EP - 194
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
SN - 0002-9637
IS - 1
ER -