TY - JOUR
T1 - Monoclonal anti-idiotypic antibodies that mimic the epitope on gp120 defined by anti-HIV-1 monoclonal antibody 0.5β
AU - Sperlagh, Melinda
AU - Stefano, Kelly
AU - Gonzalez-Scarano, Francisco
AU - Liang, Shaohong
AU - Hoxie, James
AU - Maruyama, Haruhiko
AU - Prewett, Marie
AU - Matsushita, Shuzo
AU - Herlyn, Dorothee
PY - 1993/12
Y1 - 1993/12
N2 - Objective: To develop effective, specific and safe anti-idiotypic antibody (Ab2) vaccines against HIV-1. Design: Murine monoclonal Ab2 were generated against anti-HIV-1 antibody 0.5β (Ab1), which binds to gp120, neutralizes HIV-1 and inhibits virus-induced syncytia formation. Methods: Mice were immunized with Ab1, and Ab2 were produced from immunized mice by the hybridoma technique. The Ab2 were characterized in vitro, injected into rabbits, and the anti-anti-idiotypes (Ab3) induced in the rabbits were analyzed for binding and antiviral reactivities by enzyme-linked immunosorbent assay, p24gag release and syncytia formation assays. Results: Seven Ab2 bound to the antigen-combining site of Ab1, one of which (UD7) induced Ab3 in rabbits that were Ab1-like in their binding reactivities to PB1 (recombinant gp120 fragment) or peptides of gp120, and shared idiotypes with the Ab1. Crude Ab3-containing sera specifically and effectively neutralized the virus. Conclusion: Monoclonal Ab2 UD7 has potential as a vaccine against HIV-1.
AB - Objective: To develop effective, specific and safe anti-idiotypic antibody (Ab2) vaccines against HIV-1. Design: Murine monoclonal Ab2 were generated against anti-HIV-1 antibody 0.5β (Ab1), which binds to gp120, neutralizes HIV-1 and inhibits virus-induced syncytia formation. Methods: Mice were immunized with Ab1, and Ab2 were produced from immunized mice by the hybridoma technique. The Ab2 were characterized in vitro, injected into rabbits, and the anti-anti-idiotypes (Ab3) induced in the rabbits were analyzed for binding and antiviral reactivities by enzyme-linked immunosorbent assay, p24gag release and syncytia formation assays. Results: Seven Ab2 bound to the antigen-combining site of Ab1, one of which (UD7) induced Ab3 in rabbits that were Ab1-like in their binding reactivities to PB1 (recombinant gp120 fragment) or peptides of gp120, and shared idiotypes with the Ab1. Crude Ab3-containing sera specifically and effectively neutralized the virus. Conclusion: Monoclonal Ab2 UD7 has potential as a vaccine against HIV-1.
KW - Anti-idiotypic antibody
KW - HIV-1
KW - Neutralizing antibodies
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U2 - 10.1097/00002030-199312000-00002
DO - 10.1097/00002030-199312000-00002
M3 - Article
C2 - 7506914
AN - SCOPUS:0027746280
VL - 7
SP - 1553
EP - 1559
JO - AIDS
JF - AIDS
SN - 0269-9370
IS - 12
ER -