Molecular typing of clinical and environmental isolates of Scedosporium prolificans by inter-simple-sequence-repeat polymerase chain reaction

M. Solé; J. Cano; J. L. Rodríguez-Tudela; J. Pontón; D. A. Sutton; R. Perrie; J. Gené; V. Rodríguez; J. Guarro

doi:10.1080/13693780310001600813

Molecular typing of clinical and environmental isolates of Scedosporium prolificans by inter-simple-sequence-repeat polymerase chain reaction

M. Solé, J. Cano, J. L. Rodríguez-Tudela, J. Pontón, D. A. Sutton, R. Perrie, J. Gené, V. Rodríguez, J. Guarro

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Invasive infections by Scedosporium prolificans have increased alarmingly in recent years, mainly in immunosuppressed patients. The epidemiology, pathogenesis and the natural habitat of this pathogen are practically unknown. Isolates of S. prolificans were distinguished from one another by inter-simple-sequence-repeat (ISSR) fingerprinting, a technique based on the high degree of polymorphism of the multisatellite genetic markers used. This technique was found useful for typing 84 isolates of S. prolificans from different countries and sources. The assemblage of S. prolificans isolates tested was extremely diverse, with 35 genotypes present. Several patients were found to have been infected or colonized by more than one strain. Overall, this technique facilitates the epidemiological study of S. prolificans infection.

Original language	English (US)
Pages (from-to)	293-300
Number of pages	8
Journal	Medical mycology
Volume	41
Issue number	4
DOIs	https://doi.org/10.1080/13693780310001600813
State	Published - Aug 2003

Keywords

Emerging fungi
ISSR-PCR
Molecular epidemiology
Scedosporium prolificans

ASJC Scopus subject areas

Infectious Diseases

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Molecular typing of clinical and environmental isolates of Scedosporium prolificans by inter-simple-sequence-repeat polymerase chain reaction. / Solé, M.; Cano, J.; Rodríguez-Tudela, J. L.; Pontón, J.; Sutton, D. A.; Perrie, R.; Gené, J.; Rodríguez, V.; Guarro, J.

In: Medical mycology, Vol. 41, No. 4, 08.2003, p. 293-300.

Research output: Contribution to journal › Article › peer-review

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abstract = "Invasive infections by Scedosporium prolificans have increased alarmingly in recent years, mainly in immunosuppressed patients. The epidemiology, pathogenesis and the natural habitat of this pathogen are practically unknown. Isolates of S. prolificans were distinguished from one another by inter-simple-sequence-repeat (ISSR) fingerprinting, a technique based on the high degree of polymorphism of the multisatellite genetic markers used. This technique was found useful for typing 84 isolates of S. prolificans from different countries and sources. The assemblage of S. prolificans isolates tested was extremely diverse, with 35 genotypes present. Several patients were found to have been infected or colonized by more than one strain. Overall, this technique facilitates the epidemiological study of S. prolificans infection.",

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AU - Pontón, J.

AU - Sutton, D. A.

AU - Perrie, R.

AU - Gené, J.

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AU - Guarro, J.

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AB - Invasive infections by Scedosporium prolificans have increased alarmingly in recent years, mainly in immunosuppressed patients. The epidemiology, pathogenesis and the natural habitat of this pathogen are practically unknown. Isolates of S. prolificans were distinguished from one another by inter-simple-sequence-repeat (ISSR) fingerprinting, a technique based on the high degree of polymorphism of the multisatellite genetic markers used. This technique was found useful for typing 84 isolates of S. prolificans from different countries and sources. The assemblage of S. prolificans isolates tested was extremely diverse, with 35 genotypes present. Several patients were found to have been infected or colonized by more than one strain. Overall, this technique facilitates the epidemiological study of S. prolificans infection.

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