Molecular polymorphism of Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) latent nuclear antigen: Evidence for a large repertoire of viral genotypes and dual infection with different viral genotypes

Shou Jiang Gao, Yan Jin Zhang, Jian Hong Deng, Charles S. Rabkin, Ornella Flore, Hal B. Jenson

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60 Scopus citations

Abstract

Molecular polymorphism was found in Kaposi's sarcoma-associated herpesvirus (KSHV) latent nuclear antigen (LNA), mapped to the internal repeat domain of the encoding orf73 gene, and used to develop a novel genotyping technique, KSHV LNA genotyping (KVNA-typing). KVNAtype was stable during latent and lyric viral replication in cell culture and in humans. Diverse KVNAtypes were identified in 43 specimens: 6 KSHV cell lines and 6 Kaposi's sarcoma (KS) and 4 primary effusion lymphoma (PEL) tumor samples from the United States, 15 KS tumor samples from Italy, and 12 KS tumor samples from Zambia. A single KVNAtype was detected in each of 41 specimens, and 2 KVNAtypes were detected in each of 2 KS specimens. Multifocal KS from 3 patients showed the same single KVNAtype at all sites in each patient. These results demonstrate a large repertoire of KSHV genotypes and suggest that the development of most KSs and PELs is associated with a single viral genotype.

Original languageEnglish (US)
Pages (from-to)1466-1476
Number of pages11
JournalJournal of Infectious Diseases
Volume180
Issue number5
DOIs
StatePublished - Nov 9 1999

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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