Molecular Histochemistry Identifies Peptidomic Organization and Reorganization Along Striatal Projection Units

Akitoyo Hishimoto, Hiroko Nomaru, Kenny Ye, Akira Nishi, Jihyeon Lim, Jennifer T. Aguilan, Edward Nieves, Gina Kang, Ruth Hogue Angeletti, Noboru Hiroi

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) (MALDI-IMS) provides a technical means for simultaneous analysis of precise anatomic localization and regulation of peptides. We explored the technical capability of matrix-assisted laser desorption ionization mass spectrometry for characterization of peptidomic regulation by an addictive substance along two distinct projection systems in the mouse striatum. The spatial expression patterns of substance P and proenkephalin, marker neuropeptides of two distinct striatal projection neurons, were negatively correlated at baseline. We detected 768 mass/charge (m/z) peaks whose expression levels were mostly negatively and positively correlated with expression levels of substance P and proenkephalin A (amino acids 218–228), respectively, within the dorsal striatum. After nicotine administration, there was a positive shift in correlation of mass/charge peak expression levels with substance P and proenkephalin A (218–228). Our exploratory analyses demonstrate the technical capacity of MALDI-IMS for comprehensive identification of peptidomic regulation patterns along histochemically distinguishable striatal projection pathways.

Original languageEnglish (US)
Pages (from-to)415-420
Number of pages6
JournalBiological Psychiatry
Issue number5
StatePublished - Mar 1 2016
Externally publishedYes


  • Caudate-putamen
  • In situ mass spectrometry
  • Nicotine addiction
  • Peptides
  • Peptidomics
  • Striatum

ASJC Scopus subject areas

  • Biological Psychiatry


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