Molecular heterogeneity of platelet-activating factor produced by stimulated human polymorphonuclear leukocytes

R. Neal Pinckard, Evelyn M. Jackson, Carol Hoppens, Susan T. Weintraub, Janet C. Ludwig, Linda M. McManus, Glen E. Mott

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

The molecular heterogeneity of platelet-activating factor (PAF) produced by stimulated human neutrophilic polymorphonuclear leukocytes (PMN) was assessed by both normal and reverse phase high performance liquid chromatography (HPLC). As detected by rabbit platelet stimulation, at least 5 PAF molecules were separated by HPLC. Fast atom bombardment (FAB) mass spectrometry revealed one of these PAFs was acetyl glyceryl ether phosphorylcholine (AGEPC) with a C16:0 alkyl chain in the sn-1 position. Although the structures of the remaining PAFs are unknown, two of the peaks of PAF activity had the same retention times on reverse phase HPLC as the C15- and C18-saturated alkyl chain AGEPC homologues. These studies indicate that the human PMN produces multiple molecular species of PAF.

Original languageEnglish (US)
Pages (from-to)325-332
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume122
Issue number1
DOIs
StatePublished - Jul 18 1984

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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