Molecular heterogeneity of PAF in normal human mixed saliva: quantitative mass spectral analysis after direct derivatization of PAF with pentafluorobenzoic anhydride

Donna S. Woodard, Brian L. Mealey, Cindy S. Lear, Rajiv K. Satsangi, Thomas J. Prihoda, Susan T. Weintraub, R. Neal Pinckard, Linda M. McManus

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Platelet-activating factor (PAF), a family of phospholipid autacoids with potent pro-inflammatory activities, is present in saliva. The current study has quantitated various species of PAF isolated from normal human mixed saliva. Choline-containing, sn-2 acetylated phospholipids with sn-1 ether- or ester-linked fatty alcohol/acid moieties (alkyl-PAF or acyl-PAF, respectively) were evaluated after direct defvatization with pentafluorobenzoic (PFB) anhydride. Individual species of PFB-derivatized PAF were separated by gas chromatography prior to mass spectral analysis; quantitative estimates of six different species of PAF in saliva were made by comparison to corresponding authentic, synthetic PAF standards. In each saliva sample, all six species of PAF were readily detected by this facile procedure. The predominant PAF was 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine or 16:0-alkyl-PAF (0.75 ± 0.09 pmol/ml saliva; mean ± S.E.; n = 5) which represented only 30.4 ± 1.5% of the total PAR Substantial amounts of 18:1- and 18:0-alkyl-PAF and 16:0-acyl-PAF were also identified (0.52 ± 0.07, 0.35 ± 0.06, and 0.35 ± 0.02 pmol/ml saliva, respectively). In summary, mass spectrometric analysis of PAF after direct derivatization with PFB anhydride has revealed that at least six different species of PAF are present in normal human mixed saliva. This structural diversity may represent an important aspect of homeostasis in the healthy oral cavity.

Original languageEnglish (US)
Pages (from-to)137-147
Number of pages11
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume1259
Issue number2
DOIs
StatePublished - Nov 16 1995

Keywords

  • Mass spectrometry
  • PAF
  • Platelet-activating factor
  • Saliva

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology

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