Molecular epidemiology of β-lactamase-producing enterococci

Jan E Patterson, A. Wagner, K. K. Zscheck, M. J. Zervos, B. E. Murray

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Plasmids from the first six reported β-lactamase-producing (Bla+) enterococci were compared for genetic relatedness. Bla+ enterococcal plasmids from strains isolated in Houston, Tex.; Philadelphia, Pa.; Connecticut; and Pittsburgh, Pa., had heterogeneous HaeIII and MspI-ClaI restriction endonuclease digestion patterns. A staphylococcal β-lactamase probe hybridized to all six Bla+ enterococcal plasmids, but hybridization was detected on different HaeIII and MspI-ClaI fragments of the six plasmids. An enterococcal gentamicin resistance (Gm(r)) probe hybridized to a common 3.9-kilobase HaeIII fragment from the five Gm(r) plasmids. The Houston plasmid was cross-hybridized to the other five strains, and moderate to extensive homology was demonstrated. Bla+ enterococcal plasmids from a broad geographic range are heterogeneous with respect to size and restriction endonuclease digestion patterns but contain homologous genetic material, including Bla+ and Gm(r) determinants.

Original languageEnglish (US)
Pages (from-to)302-305
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume34
Issue number2
StatePublished - 1990
Externally publishedYes

Fingerprint

Molecular Epidemiology
Enterococcus
Plasmids
Gentamicins
Digestion
DNA Restriction Enzymes
Genes

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Patterson, J. E., Wagner, A., Zscheck, K. K., Zervos, M. J., & Murray, B. E. (1990). Molecular epidemiology of β-lactamase-producing enterococci. Antimicrobial Agents and Chemotherapy, 34(2), 302-305.

Molecular epidemiology of β-lactamase-producing enterococci. / Patterson, Jan E; Wagner, A.; Zscheck, K. K.; Zervos, M. J.; Murray, B. E.

In: Antimicrobial Agents and Chemotherapy, Vol. 34, No. 2, 1990, p. 302-305.

Research output: Contribution to journalArticle

Patterson, JE, Wagner, A, Zscheck, KK, Zervos, MJ & Murray, BE 1990, 'Molecular epidemiology of β-lactamase-producing enterococci', Antimicrobial Agents and Chemotherapy, vol. 34, no. 2, pp. 302-305.
Patterson, Jan E ; Wagner, A. ; Zscheck, K. K. ; Zervos, M. J. ; Murray, B. E. / Molecular epidemiology of β-lactamase-producing enterococci. In: Antimicrobial Agents and Chemotherapy. 1990 ; Vol. 34, No. 2. pp. 302-305.
@article{55c4830247ca43969c4d03482531b9d1,
title = "Molecular epidemiology of β-lactamase-producing enterococci",
abstract = "Plasmids from the first six reported β-lactamase-producing (Bla+) enterococci were compared for genetic relatedness. Bla+ enterococcal plasmids from strains isolated in Houston, Tex.; Philadelphia, Pa.; Connecticut; and Pittsburgh, Pa., had heterogeneous HaeIII and MspI-ClaI restriction endonuclease digestion patterns. A staphylococcal β-lactamase probe hybridized to all six Bla+ enterococcal plasmids, but hybridization was detected on different HaeIII and MspI-ClaI fragments of the six plasmids. An enterococcal gentamicin resistance (Gm(r)) probe hybridized to a common 3.9-kilobase HaeIII fragment from the five Gm(r) plasmids. The Houston plasmid was cross-hybridized to the other five strains, and moderate to extensive homology was demonstrated. Bla+ enterococcal plasmids from a broad geographic range are heterogeneous with respect to size and restriction endonuclease digestion patterns but contain homologous genetic material, including Bla+ and Gm(r) determinants.",
author = "Patterson, {Jan E} and A. Wagner and Zscheck, {K. K.} and Zervos, {M. J.} and Murray, {B. E.}",
year = "1990",
language = "English (US)",
volume = "34",
pages = "302--305",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - Molecular epidemiology of β-lactamase-producing enterococci

AU - Patterson, Jan E

AU - Wagner, A.

AU - Zscheck, K. K.

AU - Zervos, M. J.

AU - Murray, B. E.

PY - 1990

Y1 - 1990

N2 - Plasmids from the first six reported β-lactamase-producing (Bla+) enterococci were compared for genetic relatedness. Bla+ enterococcal plasmids from strains isolated in Houston, Tex.; Philadelphia, Pa.; Connecticut; and Pittsburgh, Pa., had heterogeneous HaeIII and MspI-ClaI restriction endonuclease digestion patterns. A staphylococcal β-lactamase probe hybridized to all six Bla+ enterococcal plasmids, but hybridization was detected on different HaeIII and MspI-ClaI fragments of the six plasmids. An enterococcal gentamicin resistance (Gm(r)) probe hybridized to a common 3.9-kilobase HaeIII fragment from the five Gm(r) plasmids. The Houston plasmid was cross-hybridized to the other five strains, and moderate to extensive homology was demonstrated. Bla+ enterococcal plasmids from a broad geographic range are heterogeneous with respect to size and restriction endonuclease digestion patterns but contain homologous genetic material, including Bla+ and Gm(r) determinants.

AB - Plasmids from the first six reported β-lactamase-producing (Bla+) enterococci were compared for genetic relatedness. Bla+ enterococcal plasmids from strains isolated in Houston, Tex.; Philadelphia, Pa.; Connecticut; and Pittsburgh, Pa., had heterogeneous HaeIII and MspI-ClaI restriction endonuclease digestion patterns. A staphylococcal β-lactamase probe hybridized to all six Bla+ enterococcal plasmids, but hybridization was detected on different HaeIII and MspI-ClaI fragments of the six plasmids. An enterococcal gentamicin resistance (Gm(r)) probe hybridized to a common 3.9-kilobase HaeIII fragment from the five Gm(r) plasmids. The Houston plasmid was cross-hybridized to the other five strains, and moderate to extensive homology was demonstrated. Bla+ enterococcal plasmids from a broad geographic range are heterogeneous with respect to size and restriction endonuclease digestion patterns but contain homologous genetic material, including Bla+ and Gm(r) determinants.

UR - http://www.scopus.com/inward/record.url?scp=0025056530&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025056530&partnerID=8YFLogxK

M3 - Article

C2 - 2109577

AN - SCOPUS:0025056530

VL - 34

SP - 302

EP - 305

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 2

ER -