Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase δ

Chun li Yang; Long sheng Chang; Peng Zhang; Huiling Hao; Lingyun Zhu; N. Lan Toomey; Marietta Y.W.t. Lee

doi:10.1093/nar/20.4.735

Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase δ

Chun li Yang, Long sheng Chang, Peng Zhang, Huiling Hao, Lingyun Zhu, N. Lan Toomey, Marietta Y.W.t. Lee

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Abstract

The cDNA of human DNA polymerase δ was cloned. The cDNA had a length of 3.5 kb and encoded a protein of 1107 amino acid residues with a calculated molecular mass of 124 kDa. Northern blot analysis showed that the cDNA hybridized to a mRNA of 3.4 kb. Monoclonal and polyclonal antibodies to the C-terminal 20 residues specifically immunoblotted the human pol δ catalytic polypeptide. A multiple sequence alignment was constructed. This showed that human pol δ is closely related to yeast pol (EQN) and the herpes virus DNA polymerases. The levels of polδ 8 message were found to be induced concomitantly with DNA pol δ activity and DNA synthesis in serum restlmulated proliferating IMR90 cultured cells. The human pol δ8 gene was localized to chromosome 19 by Southern blotting of EcoRI digested DNA from a panel of rodent/human cell hybrids.

Original language	English (US)
Pages (from-to)	735-745
Number of pages	11
Journal	Nucleic acids research
Volume	20
Issue number	4
DOIs	https://doi.org/10.1093/nar/20.4.735
State	Published - Feb 25 1992
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase δ",

abstract = "The cDNA of human DNA polymerase δ was cloned. The cDNA had a length of 3.5 kb and encoded a protein of 1107 amino acid residues with a calculated molecular mass of 124 kDa. Northern blot analysis showed that the cDNA hybridized to a mRNA of 3.4 kb. Monoclonal and polyclonal antibodies to the C-terminal 20 residues specifically immunoblotted the human pol δ catalytic polypeptide. A multiple sequence alignment was constructed. This showed that human pol δ is closely related to yeast pol (EQN) and the herpes virus DNA polymerases. The levels of polδ 8 message were found to be induced concomitantly with DNA pol δ activity and DNA synthesis in serum restlmulated proliferating IMR90 cultured cells. The human pol δ8 gene was localized to chromosome 19 by Southern blotting of EcoRI digested DNA from a panel of rodent/human cell hybrids.",

author = "Yang, {Chun li} and Chang, {Long sheng} and Peng Zhang and Huiling Hao and Lingyun Zhu and {Lan Toomey}, N. and Lee, {Marietta Y.W.t.}",

note = "Funding Information: This work was supported by National Institutes of Health Grant GM31973 to MYWTL and in part by grants from the National Cancer Institute (CA 54323) and the Bremer and Milheim Foundation to LSC. An account of this work was presented at the Cold Spring Harbor Meeting on EukaryorJc DNA Replication in September 1991. We thank Drs A. Sugino, A. Morrison and G. Pignede for copies of their papers in press.",

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doi = "10.1093/nar/20.4.735",

language = "English (US)",

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AU - Yang, Chun li

AU - Chang, Long sheng

AU - Zhang, Peng

AU - Hao, Huiling

AU - Zhu, Lingyun

AU - Lan Toomey, N.

AU - Lee, Marietta Y.W.t.

N1 - Funding Information: This work was supported by National Institutes of Health Grant GM31973 to MYWTL and in part by grants from the National Cancer Institute (CA 54323) and the Bremer and Milheim Foundation to LSC. An account of this work was presented at the Cold Spring Harbor Meeting on EukaryorJc DNA Replication in September 1991. We thank Drs A. Sugino, A. Morrison and G. Pignede for copies of their papers in press.

PY - 1992/2/25

Y1 - 1992/2/25

N2 - The cDNA of human DNA polymerase δ was cloned. The cDNA had a length of 3.5 kb and encoded a protein of 1107 amino acid residues with a calculated molecular mass of 124 kDa. Northern blot analysis showed that the cDNA hybridized to a mRNA of 3.4 kb. Monoclonal and polyclonal antibodies to the C-terminal 20 residues specifically immunoblotted the human pol δ catalytic polypeptide. A multiple sequence alignment was constructed. This showed that human pol δ is closely related to yeast pol (EQN) and the herpes virus DNA polymerases. The levels of polδ 8 message were found to be induced concomitantly with DNA pol δ activity and DNA synthesis in serum restlmulated proliferating IMR90 cultured cells. The human pol δ8 gene was localized to chromosome 19 by Southern blotting of EcoRI digested DNA from a panel of rodent/human cell hybrids.

AB - The cDNA of human DNA polymerase δ was cloned. The cDNA had a length of 3.5 kb and encoded a protein of 1107 amino acid residues with a calculated molecular mass of 124 kDa. Northern blot analysis showed that the cDNA hybridized to a mRNA of 3.4 kb. Monoclonal and polyclonal antibodies to the C-terminal 20 residues specifically immunoblotted the human pol δ catalytic polypeptide. A multiple sequence alignment was constructed. This showed that human pol δ is closely related to yeast pol (EQN) and the herpes virus DNA polymerases. The levels of polδ 8 message were found to be induced concomitantly with DNA pol δ activity and DNA synthesis in serum restlmulated proliferating IMR90 cultured cells. The human pol δ8 gene was localized to chromosome 19 by Southern blotting of EcoRI digested DNA from a panel of rodent/human cell hybrids.

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Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase δ

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