Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase δ

Chun li Yang, Long sheng Chang, Peng Zhang, Huiling Hao, Lingyun Zhu, N. Lan Toomey, Marietta Y.W.t. Lee

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Abstract

The cDNA of human DNA polymerase δ was cloned. The cDNA had a length of 3.5 kb and encoded a protein of 1107 amino acid residues with a calculated molecular mass of 124 kDa. Northern blot analysis showed that the cDNA hybridized to a mRNA of 3.4 kb. Monoclonal and polyclonal antibodies to the C-terminal 20 residues specifically immunoblotted the human pol δ catalytic polypeptide. A multiple sequence alignment was constructed. This showed that human pol δ is closely related to yeast pol (EQN) and the herpes virus DNA polymerases. The levels of polδ 8 message were found to be induced concomitantly with DNA pol δ activity and DNA synthesis in serum restlmulated proliferating IMR90 cultured cells. The human pol δ8 gene was localized to chromosome 19 by Southern blotting of EcoRI digested DNA from a panel of rodent/human cell hybrids.

Original languageEnglish (US)
Pages (from-to)735-745
Number of pages11
JournalNucleic acids research
Volume20
Issue number4
DOIs
StatePublished - Feb 25 1992

ASJC Scopus subject areas

  • Genetics

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