Molecular cloning of cellular genes encoding retinoblastoma-associated proteins: Identification of a gene with properties of the transcription factor E2F

Bei Shan, Xueliang Zhu, Phang Lang Chen, Tim Durfee, Yanzhu Yang, Dave Sharp, Wen Hwa Lee

Research output: Contribution to journalArticle

266 Scopus citations

Abstract

The retinoblastoma protein interacts with a number of cellular proteins to form complexes which are probably crucial for its normal physiological function. To identify these proteins, we isolated nine distinct clones by direct screening of cDNA expression libraries using purified RB protein as a probe. One of these clones, Ap12, is expressed predominantly at the G1-S boundary and in the S phase of the cell cycle. The nucleotide sequence of Ap12 has features characteristic of transcription factors. The C-terminal region binds to unpbosphorylated RB in regions similar to those to which T antigen binds and contains a transactivation domain. A region containing a potential leucine zipper flanked by basic residues is able to bind an E2F recognition sequence specifically. Expression of Ap12 in mammalian cells significantly enhances E2F-dependent transcriptional activity. These results suggest that Ap12 encodes a protein with properties known to be characteristic of transcription factor E2F.

Original languageEnglish (US)
Pages (from-to)5620-5631
Number of pages12
JournalMolecular and cellular biology
Volume12
Issue number12
DOIs
StatePublished - Dec 1992

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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