TY - JOUR
T1 - Molecular cloning and functional characterisation of a glucose transporter, CaHGT1, of Candida albicans
AU - Varma, Archana
AU - Singh, Brij Bhan
AU - Karnani, Neerja
AU - Lichtenberg-Fraté, Hella
AU - Höfer, Milan
AU - Magee, B. B.
AU - Prasad, Rajendra
N1 - Funding Information:
The work in the laboratory of R.P. was supported by grants from the Department of Biotechnology (DBT) (BT/R and D/15/02/94), Department of Science and Technology (SP/SO/D57/97), Council of Scientific, Industrial Research (60(0028)/98-EMR-II), India and from the collaborative grant to R.P. and M.H. from the European Commission (TS3-CT94-0279). The work in the laboratory of B.B.M. was supported by a grant from the National Institute of Allergy and Infectious Diseases (AI16567). A.V. and N.K. are recipients of senior research fellowships awarded by the University Grants Commission, Government of India.
PY - 2000/1/1
Y1 - 2000/1/1
N2 - We have cloned the first glucose transporter CaHGT1 (Candida albicans high-affinity glucose transporter) of a pathogenic yeast, Candida albicans. The DNA sequence (GenBank accession number Y16834) analysis revealed an ORF encoding a novel protein of 545 amino acids with a predicted molecular mass of 60.67 kDa. The putative protein with 12 transmembrane domains has 51% identity with Kluyveromyces lactis high-affinity glucose transporter, HGT1. The protein signatures which are conserved and distinctive of the sugar transporters belonging to the major facilitator superfamily (MFS) were also found in CaHgt1p. When heterologously expressed, the ORF functionally complemented a mutant strain of Saccharomyces cerevisiae RE700A which was deleted in seven hexose transporter genes and thus was unable to grow or transport glucose. The expression of CaHGT1 in C. albicans showed a transcript of 1.6 kb which was enhanced in response to the human steroid hormone progesterone. Interestingly, the transcript levels were also enhanced in the presence of drugs, e.g. cycloheximide, chloramphenicol and benomyl. The results suggest that CaHGT1, which encodes a MFS protein, could be linked to the drug resistance phenomenon in C. albicans. Copyright (C) 2000 Federation of European Microbiological Societies.
AB - We have cloned the first glucose transporter CaHGT1 (Candida albicans high-affinity glucose transporter) of a pathogenic yeast, Candida albicans. The DNA sequence (GenBank accession number Y16834) analysis revealed an ORF encoding a novel protein of 545 amino acids with a predicted molecular mass of 60.67 kDa. The putative protein with 12 transmembrane domains has 51% identity with Kluyveromyces lactis high-affinity glucose transporter, HGT1. The protein signatures which are conserved and distinctive of the sugar transporters belonging to the major facilitator superfamily (MFS) were also found in CaHgt1p. When heterologously expressed, the ORF functionally complemented a mutant strain of Saccharomyces cerevisiae RE700A which was deleted in seven hexose transporter genes and thus was unable to grow or transport glucose. The expression of CaHGT1 in C. albicans showed a transcript of 1.6 kb which was enhanced in response to the human steroid hormone progesterone. Interestingly, the transcript levels were also enhanced in the presence of drugs, e.g. cycloheximide, chloramphenicol and benomyl. The results suggest that CaHGT1, which encodes a MFS protein, could be linked to the drug resistance phenomenon in C. albicans. Copyright (C) 2000 Federation of European Microbiological Societies.
KW - CaHGT1
KW - Candida albicans
KW - Glucose transporter
KW - Major facilitator superfamily
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U2 - 10.1016/S0378-1097(99)00558-3
DO - 10.1016/S0378-1097(99)00558-3
M3 - Article
C2 - 10612724
AN - SCOPUS:0033989919
VL - 182
SP - 15
EP - 21
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 1
ER -