Molecular cloning and functional characterisation of a glucose transporter, CaHGT1, of Candida albicans

Archana Varma, Brij B Singh, Neerja Karnani, Hella Lichtenberg-Fraté, Milan Höfer, B. B. Magee, Rajendra Prasad

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have cloned the first glucose transporter CaHGT1 (Candida albicans high-affinity glucose transporter) of a pathogenic yeast, Candida albicans. The DNA sequence (GenBank accession number Y16834) analysis revealed an ORF encoding a novel protein of 545 amino acids with a predicted molecular mass of 60.67 kDa. The putative protein with 12 transmembrane domains has 51% identity with Kluyveromyces lactis high-affinity glucose transporter, HGT1. The protein signatures which are conserved and distinctive of the sugar transporters belonging to the major facilitator superfamily (MFS) were also found in CaHgt1p. When heterologously expressed, the ORF functionally complemented a mutant strain of Saccharomyces cerevisiae RE700A which was deleted in seven hexose transporter genes and thus was unable to grow or transport glucose. The expression of CaHGT1 in C. albicans showed a transcript of 1.6 kb which was enhanced in response to the human steroid hormone progesterone. Interestingly, the transcript levels were also enhanced in the presence of drugs, e.g. cycloheximide, chloramphenicol and benomyl. The results suggest that CaHGT1, which encodes a MFS protein, could be linked to the drug resistance phenomenon in C. albicans. Copyright (C) 2000 Federation of European Microbiological Societies.

Original languageEnglish (US)
Pages (from-to)15-21
Number of pages7
JournalFEMS Microbiology Letters
Volume182
Issue number1
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

Fingerprint

Facilitative Glucose Transport Proteins
Molecular Cloning
Candida albicans
Open Reading Frames
Proteins
Monosaccharide Transport Proteins
Benomyl
Kluyveromyces
Nucleic Acid Databases
Chloramphenicol
Cycloheximide
Drug Resistance
Progesterone
Saccharomyces cerevisiae
Yeasts
Steroids
Hormones
Amino Acids
Glucose
Pharmaceutical Preparations

Keywords

  • CaHGT1
  • Candida albicans
  • Glucose transporter
  • Major facilitator superfamily

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Molecular cloning and functional characterisation of a glucose transporter, CaHGT1, of Candida albicans. / Varma, Archana; Singh, Brij B; Karnani, Neerja; Lichtenberg-Fraté, Hella; Höfer, Milan; Magee, B. B.; Prasad, Rajendra.

In: FEMS Microbiology Letters, Vol. 182, No. 1, 01.01.2000, p. 15-21.

Research output: Contribution to journalArticle

Varma, Archana ; Singh, Brij B ; Karnani, Neerja ; Lichtenberg-Fraté, Hella ; Höfer, Milan ; Magee, B. B. ; Prasad, Rajendra. / Molecular cloning and functional characterisation of a glucose transporter, CaHGT1, of Candida albicans. In: FEMS Microbiology Letters. 2000 ; Vol. 182, No. 1. pp. 15-21.
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