Molecular Cloning and Characterization of PELP1, a Novel Human Coregulator of Estrogen Receptor α

Ratna K Vadlamudi, Rui An Wang, Abhijit Mazumdar, Yoon Sok Kim, Jaekyoon Shin, Aysegul Sahin, Rakesh Kumar

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Nuclear hormone receptors (NRs) are transcription factors whose activity is regulated by ligands and by coactivators or corepressors. We report the characterization of a new NR coregulator: proline-, glutamic acid-, leucine-rich protein 1 (PELP1), a novel human protein that comprises 1,282 amino acids and is localized on chromosome 17. The primary structure of PELP1 consists of several motifs present in most transcriptional regulators including nine NR-interacting boxes (LXXLL motifs), a zinc finger, and glutamic acid- and proline-rich regions. We demonstrate that PELP1 is a coactivator of estrogen receptor α (ERα). PELP1 enhances 17β-estradiol-dependent transcriptional activation from the estrogen response element in a dose-dependent manner. PELP1 interacts with ERα and also with general transcriptional coactivators p300 and cAMP response element-binding protein-binding protein. PELP1 was differentially expressed in various human and murine tissues with the highest expression levels in the testes, mammary glands, and brain. We also provide evidence supporting the developmental regulation of PELP1 expression in murine mammary glands, the detectable expression of PELP1 in human mammary cancer cell lines, and the enhanced expression of PELP1 in human breast tumors. These findings suggest that PELP1 is a novel coregulator of ERα and may have a role in breast cancer tumorigenesis.

Original languageEnglish (US)
Pages (from-to)38272-38279
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number41
DOIs
StatePublished - Oct 12 2001
Externally publishedYes

Fingerprint

Cloning
Molecular Cloning
Estrogen Receptors
Cytoplasmic and Nuclear Receptors
Human Mammary Glands
Breast Neoplasms
human PELP1 protein
Cyclic AMP Response Element-Binding Protein
Co-Repressor Proteins
Chromosomes, Human, Pair 17
Zinc Fingers
Response Elements
Chromosomes
Proline
Protein Binding
Transcriptional Activation
Testis
Zinc
Tumors
Glutamic Acid

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular Cloning and Characterization of PELP1, a Novel Human Coregulator of Estrogen Receptor α. / Vadlamudi, Ratna K; Wang, Rui An; Mazumdar, Abhijit; Kim, Yoon Sok; Shin, Jaekyoon; Sahin, Aysegul; Kumar, Rakesh.

In: Journal of Biological Chemistry, Vol. 276, No. 41, 12.10.2001, p. 38272-38279.

Research output: Contribution to journalArticle

Vadlamudi, Ratna K ; Wang, Rui An ; Mazumdar, Abhijit ; Kim, Yoon Sok ; Shin, Jaekyoon ; Sahin, Aysegul ; Kumar, Rakesh. / Molecular Cloning and Characterization of PELP1, a Novel Human Coregulator of Estrogen Receptor α. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 41. pp. 38272-38279.
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