Molecular cloning and characterization of an adherence-related operon of Mycoplasma genitalium

S. P. Reddy, W. G. Rasmussen, J. B. Baseman

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Adhesins and adhesin-related accessory proteins of pathogenic mycoplasmas are required for cytadherence and the subsequent development of disease pathology. The classic example has been Mycoplasma pneumoniae, which causes primary atypical pneumonia in humans. Mutants of M. pneumoniae defective in adhesins (P1 and P30) or in adherence-accessory proteins (HMW1 through HMW4) are unable to colonize host tissues and are avirulent. Mycoplasma genitalium, implicated in nongonococcal, nonchlamydial urethritis, pneumonia, arthritis, and AIDS progression, was found to encode a 140-kDa adhesin that shared both DNA and protein sequence similarities with P1, a major adhesin of M. pneumoniae. In this report, we show that M. genitalium possesses additional homolog sequences to well-characterized adherence-related genes and proteins of M. pneumoniae. The M. genitalium homologs are designated P32 and P69 and correspond to P30 and HMW3 of M. pneumoniae, respectively (J. B. Baseman, p. 243-259, in S. Rottem and I. Kahane, ed., Subcellular biochemistry, vol. 20. Mycoplasma cell membranes, 1993, and D.C. Krause, D. K. Leith, R. M. Wilson, and J. B. Baseman, Infect. Immun. 35:809-817, 1982). Interestingly, the operon-like organizations of P32 and P69 in the M. genitalium genome are similar to the organizations of P30 and HMW3 genes of M. pneumoniae, suggesting that the conservation of these adherence-related genes and proteins might have occurred through horizontal gene transfer events originating from an ancestral gene family.

Original languageEnglish (US)
Pages (from-to)5943-5951
Number of pages9
JournalJournal of Bacteriology
Volume177
Issue number20
StatePublished - 1995

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Mycoplasma genitalium
Mycoplasma pneumoniae
Molecular Cloning
Operon
Mycoplasma
Proteins
Mycoplasma Pneumonia
Horizontal Gene Transfer
Urethritis
Sequence Homology
Biochemistry
Genes
Arthritis
Pneumonia
Acquired Immunodeficiency Syndrome
Cell Membrane
Genome
Pathology

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Reddy, S. P., Rasmussen, W. G., & Baseman, J. B. (1995). Molecular cloning and characterization of an adherence-related operon of Mycoplasma genitalium. Journal of Bacteriology, 177(20), 5943-5951.

Molecular cloning and characterization of an adherence-related operon of Mycoplasma genitalium. / Reddy, S. P.; Rasmussen, W. G.; Baseman, J. B.

In: Journal of Bacteriology, Vol. 177, No. 20, 1995, p. 5943-5951.

Research output: Contribution to journalArticle

Reddy, SP, Rasmussen, WG & Baseman, JB 1995, 'Molecular cloning and characterization of an adherence-related operon of Mycoplasma genitalium', Journal of Bacteriology, vol. 177, no. 20, pp. 5943-5951.
Reddy, S. P. ; Rasmussen, W. G. ; Baseman, J. B. / Molecular cloning and characterization of an adherence-related operon of Mycoplasma genitalium. In: Journal of Bacteriology. 1995 ; Vol. 177, No. 20. pp. 5943-5951.
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