Molecular characterization of a copper transport protein in S. cerevisiae: An unexpected role for copper in iron transport

Andrew Dancis, Daniel S. Yuan, David Haile, Candice Askwith, David Eide, Charles Moehle, Jerry Kaplan, Richard D. Klausner

Research output: Contribution to journalArticle

525 Scopus citations

Abstract

We report the identification and characterization of CTR1, a gene in the yeast S. cerevisiae that encodes a multispanning plasma membrane protein specifically required for high affinity copper transport into the cell. The predicted protein contains a methionine- and serine-rich domain that includes 11 examples of the sequence Met-X2-Met, a motif noted in proteins involved in bacterial copper metabolism. CTR1 mutants and deletion strains have profound deficiency in ferrous iron uptake, thus revealing a requirement for copper in mediating ferrous transport into the cell. Genetic evidence suggests that the target for this requirement is the FET3 gene (detailed in a companion study), predicted to encode a copper-containing protein that acts as a cytosolic ferro-oxidase. These findings provide an unexpected mechanistic link between the uptake of copper and iron.

Original languageEnglish (US)
Pages (from-to)393-402
Number of pages10
JournalCell
Volume76
Issue number2
DOIs
StatePublished - Jan 28 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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