Molecular and phenotypic characterization of phialemonium and lecythophora isolates from clinical samples

H. Perdomo; D. A. Sutton; D. García; A. W. Fothergill; J. Gené; J. Cano; R. C. Summerbell; M. G. Rinaldi; J. Guarro

doi:10.1128/JCM.01979-10

Molecular and phenotypic characterization of phialemonium and lecythophora isolates from clinical samples

H. Perdomo, D. A. Sutton, D. García, A. W. Fothergill, J. Gené, J. Cano, R. C. Summerbell, M. G. Rinaldi, J. Guarro

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Abstract

Several members of the fungal genera Phialemonium and Lecythophora are occasional agents of severe human and animal infections. These species are difficult to identify, and relatively little is known about their frequency in the clinical setting. The objective of this study was to characterize morphologically and molecularly, on the basis of the analysis of large-subunit ribosomal DNA sequences, a set of 68 clinical isolates presumed to belong to these genera. A total of 59 isolates were determined to be Phialemonium species (n = 32) or a related Cephalotheca species (n = 6) or Lecythophora species (n = 20) or a related Coniochaeta species (n = 1). Nine isolates identified to be Acremonium spp. or Phaeoacremonium spp. were excluded from further study. The most common species were Phialemonium obovatum and Phialemonium curvatum, followed by Lecythophora hoffmannii, Cephalotheca foveolata, and Lecythophora mutabilis.

Original language	English (US)
Pages (from-to)	1209-1216
Number of pages	8
Journal	Journal of clinical microbiology
Volume	49
Issue number	4
DOIs	https://doi.org/10.1128/JCM.01979-10
State	Published - Apr 2011

ASJC Scopus subject areas

Microbiology (medical)

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title = "Molecular and phenotypic characterization of phialemonium and lecythophora isolates from clinical samples",

abstract = "Several members of the fungal genera Phialemonium and Lecythophora are occasional agents of severe human and animal infections. These species are difficult to identify, and relatively little is known about their frequency in the clinical setting. The objective of this study was to characterize morphologically and molecularly, on the basis of the analysis of large-subunit ribosomal DNA sequences, a set of 68 clinical isolates presumed to belong to these genera. A total of 59 isolates were determined to be Phialemonium species (n = 32) or a related Cephalotheca species (n = 6) or Lecythophora species (n = 20) or a related Coniochaeta species (n = 1). Nine isolates identified to be Acremonium spp. or Phaeoacremonium spp. were excluded from further study. The most common species were Phialemonium obovatum and Phialemonium curvatum, followed by Lecythophora hoffmannii, Cephalotheca foveolata, and Lecythophora mutabilis.",

author = "H. Perdomo and Sutton, {D. A.} and D. Garc{\'i}a and Fothergill, {A. W.} and J. Gen{\'e} and J. Cano and Summerbell, {R. C.} and Rinaldi, {M. G.} and J. Guarro",

note = "Funding Information: Acknowledgments This research has been done in the frame of the MINECO research projects EOSLIDE (IPT-2011-1234-310000) and GEOSIR (AYA2010-17448). PJG acknowledges the Banting Postdoctoral Fellowship (Canadian Government). KFTs research is supported by an NSERC Discovery Grant. It is a contribution for the CEI Campus Moncloa. Funding Information: This research has been done in the frame of the MINECO research projects EOSLIDE (IPT-2011-1234-310000) and GEOSIR (AYA2010-17448). PJG acknowledges the Banting Postdoctoral Fellowship (Canadian Government). KFTs research is supported by an NSERC Discovery Grant. It is a contribution for the CEI Campus Moncloa.",

year = "2011",

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doi = "10.1128/JCM.01979-10",

language = "English (US)",

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T1 - Molecular and phenotypic characterization of phialemonium and lecythophora isolates from clinical samples

AU - Perdomo, H.

AU - Sutton, D. A.

AU - García, D.

AU - Fothergill, A. W.

AU - Gené, J.

AU - Cano, J.

AU - Summerbell, R. C.

AU - Rinaldi, M. G.

AU - Guarro, J.

N1 - Funding Information: Acknowledgments This research has been done in the frame of the MINECO research projects EOSLIDE (IPT-2011-1234-310000) and GEOSIR (AYA2010-17448). PJG acknowledges the Banting Postdoctoral Fellowship (Canadian Government). KFTs research is supported by an NSERC Discovery Grant. It is a contribution for the CEI Campus Moncloa. Funding Information: This research has been done in the frame of the MINECO research projects EOSLIDE (IPT-2011-1234-310000) and GEOSIR (AYA2010-17448). PJG acknowledges the Banting Postdoctoral Fellowship (Canadian Government). KFTs research is supported by an NSERC Discovery Grant. It is a contribution for the CEI Campus Moncloa.

PY - 2011/4

Y1 - 2011/4

N2 - Several members of the fungal genera Phialemonium and Lecythophora are occasional agents of severe human and animal infections. These species are difficult to identify, and relatively little is known about their frequency in the clinical setting. The objective of this study was to characterize morphologically and molecularly, on the basis of the analysis of large-subunit ribosomal DNA sequences, a set of 68 clinical isolates presumed to belong to these genera. A total of 59 isolates were determined to be Phialemonium species (n = 32) or a related Cephalotheca species (n = 6) or Lecythophora species (n = 20) or a related Coniochaeta species (n = 1). Nine isolates identified to be Acremonium spp. or Phaeoacremonium spp. were excluded from further study. The most common species were Phialemonium obovatum and Phialemonium curvatum, followed by Lecythophora hoffmannii, Cephalotheca foveolata, and Lecythophora mutabilis.

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Molecular and phenotypic characterization of phialemonium and lecythophora isolates from clinical samples

Abstract

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