Molecular and immunohistochemical analysis of P53 in phaeochromocytoma

P. L.M. Dahia; R. C.T. Aguiar; A. M. Tsanaclis; I. Bendit; N. M.A. Abelin; S. P.A. Toledo

doi:10.1038/bjc.1995.487

Molecular and immunohistochemical analysis of P53 in phaeochromocytoma

P. L.M. Dahia, R. C.T. Aguiar, A. M. Tsanaclis, I. Bendit, N. M.A. Abelin, S. P.A. Toledo

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

We searched for mutations of the p53 gene in 25 phaeochromocytomas using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis of the entire conserved region of the gene, encompassing exons 4-8; expression of the p53 protein was assessed by immunohistochemistry. No mutations were found, while a polymorphism in codon 72 was observed. Immunohistochemistry revealed nuclear p53 overexpression in one tumour sample. We conclude that mutations of the ‘hotspot’ region of the p53 gene do not seem to play a role in the pathogenesis of phaeochromocytoma.

Original language	English (US)
Pages (from-to)	1211-1213
Number of pages	3
Journal	British Journal of Cancer
Volume	72
Issue number	5
DOIs	https://doi.org/10.1038/bjc.1995.487
State	Published - Nov 1995
Externally published	Yes

Keywords

Immunohistochemistry
Mokcular screening
Mutation
P53 gene
Phaeochromocytoma
Polymorphism

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1038/bjc.1995.487

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@article{bfea308da78047c99231145122fa1ba7,

title = "Molecular and immunohistochemical analysis of P53 in phaeochromocytoma",

abstract = "We searched for mutations of the p53 gene in 25 phaeochromocytomas using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis of the entire conserved region of the gene, encompassing exons 4-8; expression of the p53 protein was assessed by immunohistochemistry. No mutations were found, while a polymorphism in codon 72 was observed. Immunohistochemistry revealed nuclear p53 overexpression in one tumour sample. We conclude that mutations of the {\textquoteleft}hotspot{\textquoteright} region of the p53 gene do not seem to play a role in the pathogenesis of phaeochromocytoma.",

keywords = "Immunohistochemistry, Mokcular screening, Mutation, P53 gene, Phaeochromocytoma, Polymorphism",

author = "Dahia, {P. L.M.} and Aguiar, {R. C.T.} and Tsanaclis, {A. M.} and I. Bendit and Abelin, {N. M.A.} and Toledo, {S. P.A.}",

note = "Funding Information: The authors are indebted to Professor A Grossman for providing the conditions for analysis of exon 4 and for his helpful comments on the manuscript, to Dr SL Chew for critical review of the manuscript and to Drs M Ezabella, C Hayashida. B Mendonga, C Longui, D Malerbi, J Praxedes, H Bernardes and A Pereira for their assistance with some of the tumour specimens. This work was supported in part by Fundaq&o Faculdade de Medicina. CAPES and FAPESP (No. 92 2548-5).",

year = "1995",

month = nov,

doi = "10.1038/bjc.1995.487",

language = "English (US)",

volume = "72",

pages = "1211--1213",

journal = "British Journal of Cancer",

issn = "0007-0920",

publisher = "Springer Nature",

number = "5",

}

TY - JOUR

T1 - Molecular and immunohistochemical analysis of P53 in phaeochromocytoma

AU - Dahia, P. L.M.

AU - Aguiar, R. C.T.

AU - Tsanaclis, A. M.

AU - Bendit, I.

AU - Abelin, N. M.A.

AU - Toledo, S. P.A.

N1 - Funding Information: The authors are indebted to Professor A Grossman for providing the conditions for analysis of exon 4 and for his helpful comments on the manuscript, to Dr SL Chew for critical review of the manuscript and to Drs M Ezabella, C Hayashida. B Mendonga, C Longui, D Malerbi, J Praxedes, H Bernardes and A Pereira for their assistance with some of the tumour specimens. This work was supported in part by Fundaq&o Faculdade de Medicina. CAPES and FAPESP (No. 92 2548-5).

PY - 1995/11

Y1 - 1995/11

N2 - We searched for mutations of the p53 gene in 25 phaeochromocytomas using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis of the entire conserved region of the gene, encompassing exons 4-8; expression of the p53 protein was assessed by immunohistochemistry. No mutations were found, while a polymorphism in codon 72 was observed. Immunohistochemistry revealed nuclear p53 overexpression in one tumour sample. We conclude that mutations of the ‘hotspot’ region of the p53 gene do not seem to play a role in the pathogenesis of phaeochromocytoma.

AB - We searched for mutations of the p53 gene in 25 phaeochromocytomas using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis of the entire conserved region of the gene, encompassing exons 4-8; expression of the p53 protein was assessed by immunohistochemistry. No mutations were found, while a polymorphism in codon 72 was observed. Immunohistochemistry revealed nuclear p53 overexpression in one tumour sample. We conclude that mutations of the ‘hotspot’ region of the p53 gene do not seem to play a role in the pathogenesis of phaeochromocytoma.

KW - Immunohistochemistry

KW - Mokcular screening

KW - Mutation

KW - P53 gene

KW - Phaeochromocytoma

KW - Polymorphism

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DO - 10.1038/bjc.1995.487

M3 - Article

C2 - 7577469

AN - SCOPUS:0028789492

SN - 0007-0920

VL - 72

SP - 1211

EP - 1213

JO - British Journal of Cancer

JF - British Journal of Cancer

IS - 5

ER -

Molecular and immunohistochemical analysis of P53 in phaeochromocytoma

Abstract

Keywords

ASJC Scopus subject areas

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