Molecular and immunohistochemical analysis of P53 in phaeochromocytoma

P. L.M. Dahia, R. C.T. Aguiar, A. M. Tsanaclis, I. Bendit, N. M.A. Abelin, S. P.A. Toledo

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

We searched for mutations of the p53 gene in 25 phaeochromocytomas using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis of the entire conserved region of the gene, encompassing exons 4-8; expression of the p53 protein was assessed by immunohistochemistry. No mutations were found, while a polymorphism in codon 72 was observed. Immunohistochemistry revealed nuclear p53 overexpression in one tumour sample. We conclude that mutations of the ‘hotspot’ region of the p53 gene do not seem to play a role in the pathogenesis of phaeochromocytoma.

Original languageEnglish (US)
Pages (from-to)1211-1213
Number of pages3
JournalBritish Journal of Cancer
Volume72
Issue number5
DOIs
StatePublished - Nov 1995
Externally publishedYes

Keywords

  • Immunohistochemistry
  • Mokcular screening
  • Mutation
  • P53 gene
  • Phaeochromocytoma
  • Polymorphism

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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