Molecular anatomy of the recombination mediator function of Saccharomyces cerevisiae Rad5

Changhyun Seong, Michael G. Sehorn, Iben Plate, Idina Shi, Binwei Song, Peter Chi, Uffe Mortensen, Patrick Sung, Lumir Krejci

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


A helical filament of Rad51 on single-strand DNA (ssDNA), called the presynaptic filament, catalyzes DNA joint formation during homologous recombination. Rad52 facilitates presynaptic filament assembly, and this recombination mediator activity is thought to rely on the interactions of Rad52 with Rad51, the ssDNA-binding protein RPA, and ssDNA. The N-terminal region of Rad52, which has DNA binding activity and an oligomeric structure, is thought to be crucial for mediator activity and recombination. Unexpectedly, we find that the C-terminal region of Rad52 also harbors a DNA binding function. Importantly, the Rad52 C-terminal portion alone can promote Rad51 presynaptic filament assembly. The middle portion of Rad52 associates with DNA-bound RPA and contributes to the recombination mediator activity. Accordingly, expression of a protein species that harbors the middle and C-terminal regions of Rad52 in the rad52 Δ327 background enhances the association of Rad51 protein with a HO-made DNA double-strand break and partially complements the methyl-methane sulfonate sensitivity of the mutant cells. Our results provide a mechanistic framework for rationalizing the multi-faceted role of Rad52 in recombination and DNA repair.

Original languageEnglish (US)
Pages (from-to)12166-12174
Number of pages9
JournalJournal of Biological Chemistry
Issue number18
StatePublished - May 2 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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